上海中乔新舟生物科技有限公司

铁是在许多生物过程中起重要作用的元素。铁与几种蛋白质结合，例如红细胞中的氧运输血红蛋白和肌肉中的氧储存蛋白肌红蛋白。它也被铁蛋白和含铁血黄素储存。无机铁有助于许多酶（如固氮酶）的铁硫簇中的氧化还原反应。在该比色测定中，铁载体蛋白将在酸性条件下将铁解离成溶液。在它们被还原成亚铁离子后，这些离子与色原反应产生稳定的有色络合物并在590nm处产生吸光度。由此形成的颜色强度与样品中铁的浓度成正比。缓冲液中包含特定的螯合物化学物质以阻止铜离子干扰。

产品使用说明:仅供科研研究使用

货号	8448
产地	美国
缩写	IR
规格	100 tests
用途	科研
保存	-20°C
运输	干冰

1.）Ferritinophagy is required for the induction of ferroptosis by the bromodomain protein BRD4 inhibitor (+)-JQ1 in cancer cells，Cell Death Dis. 2019 May; 10(5): 331.Published online 2019 Apr 15. doi: 10.1038/s41419-019-1564-7

Question:

Hello, I wanted some clarification for part B: preparation of test samples for the Iron Assay Kit (Catalog #8448). I am confused what it means by " 4 volumes of the assay buffer", and "93 volumes of assay buffer (8448a), 2 volumes reductant (8448e) and 5 volumes sample reagents (8448d)." Does volumes just mean any unit of volume as long as that ratio is used? Thank you, Lauren Nardacci

Answers:

Posted by Manoj Sharma on Wednesday, January 6, 2016

Hi Lauren,
We have revised the preparation as follows:
B. Preparation of test samples
1. One volume of cells or tissues can be homogenized in 4 volumes of the assay buffer (8448a). Centrifuge the samples at 13,000 ×g for 10 minutes to remove insoluble material.
2. Prepare a fresh working buffer by mixing assay buffer (8448a), reductant (8448e) and sample reagents (8448d) at 93:2:5 ratio (volume ratio). (e.g., 930 μL mixing assay buffer, 20 μL reductant, and 50 μL sample reagents)
3. Mix sample (cell lysate or serum) with equal volume of working buffer.
4. Centrifuge the mixed sample 13,000 ×g for 5 minutes and take the supernatant as the test samples for the iron assay.
5. Test samples should be serial diluted to make sure the readings are within the standard curve range. Prepare test samples to a final volume of 50 μL/well on the 96-well flat bottom plate.

The product sheet is updated as well.

let us know if you have any further questions.

SCIENCELL TEAM

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公司简介 / Company profile

上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
上海中乔新舟生物科技有限公司（官网：www.zqx...

联系我们 / Contact

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邮　箱：sales@zqxzbio.com
邮　编：200439
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