0.2％肝素描述：

      Heparin is a 10-12 kDa polysaccharide produced in mast cells. Heparin inhibits blood coagulation through binding and activation of anti-thrombin [1] and acts as a cofactor to basic Fibroblast Growth Factor-2 (bFGF) [2, 3, 4].

        The effect of heparin on cell growth and morphology in vitro is cell-type dependent and may promote or inhibit cell proliferation. Culture media may be supplemented with heparin solution (HEP) at 1:1000-1:100 (0.4 – 4.0 U/mL). The optimal concentration should be determined for individual cell types and culture conditions.

Concentration

2 mg/mL heparin sodium salt (from porcine intestinal mucosa) in Dulbecco’s Phosphate-Buffered Saline, sterile-filtered. Potency: 200 Units per milligram*.

*Units are USP (United States Pharmacopeia units) and indicate heparin potency by weight. Units are not evaluated by ScienCell but are provided by supplier.

产品使用说明：仅供科研研究使用

[2] Rapraeger AC, Krufka A and Olwin BB. (1991) "Requirement of heparin sulfate for bFGF-mediated fibroflast growth and myoblast differentiation". Science. 252(5013):1705-8.

[3] Roghani M, Mansukhani A, et al. (1994) "Heparin increases the affinity of basic fibroblast growth factor for its receptor but is not required for binding". J Biol Chem. 269(6):3976-84.

[4] Sasisekharan R, Ernst S and Venkataraman G. (1997) "On the regulation of fibroblast growth factor activity by heparin-like glycosaminoglycans". Angiogenesis, 1(1):45-54.

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