肾小球内皮细胞是一类特殊微血管类型的细胞，能够调节肾小球过滤。它是组成过滤屏障内壁的重要部分，与肾小球的病理生理过程有关。肾小球内皮细胞能合成必要的生物活性分子，它的这种基本活性会在致炎和致血栓物质的刺激下慢慢增强。肾小球内皮细胞的受损显著影响着肾小球疾病的进展和修复过程。当肾小球损害严重时，内皮受损无法新生血管，硬化代替受损区域。肾小球内皮细胞受损后不可避免地会影响系膜细胞和上皮细胞并有可能通过它们的相互作用改变肾脏病的进展。因为这类细胞培养、克隆、增殖都比较困难，其生物学特点至今知之甚少。

       人肾小球内皮细胞(HRGEC)提取于人肾组织，原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。

推荐培养基：内皮细胞专用全能培养基ECM（NO.1001） 

产品使用说明：仅供科研研究使用，禁止用于人或动物的体外诊断。

4.) Advani A, Kelly DJ, Advani SL, Cox AJ, Thai K, Zhang Y, White KE, Gow RM, Marshall SM, Steer BM, Marsden PA, Rakoczy PE, Gilbert RE. (2007) "Role of VEGF in maintaining renal structure and function under normotensive and hypertensive conditions." Proc Natl Acad Sci USA. 104: 14448-53.

5.） Nangaku, M., Shankland, S. J., Couser, W. G. and Johnson, R. J. (1998) A new model of renal microvascular injury. Curr Opin Nephrol Hypertens 7(4):457-62. 

6.） Kester, M., Nowinski, R. J., Holthofer, H., Marsden, P. A. and Dunn, M. J. (1994) Characterization of platelet-activating factor synthesis in glomerular endothelial cell lines. Kidney Int 46(5):1404-12.
7.） Lee, L. K., Meyer, T. W., Pollock, A. S. and Lovett, D. H. (1995) Endothelial cell injury initiates glomerular sclerosis in the rat remnant kidney. J Clin Invest 96(2):953-64.
8.） Yamanaka, N. and Shimizu, A. (1999) Role of glomerular endothelial damage in progressive renal disease. Kidney Blood Press Res 22(1-2):13-20.

Question:

We bought a tube of Human Renal Glomerular Endothelial Cells (cat#4000) recently. I started culture immediately after receiving the tube. I started with two T75 and they were confluent the next day. I split them 1:3 and then another 1:3 split. Then I ran flow to test the suface marker and found less than 50% of the cells were CD31+. I can send my flow data for your reference. Do you have any explanation for this? The cells were not pure or they quickly lost endothelial phenotype upon culturing. I can’t use these cells with less than 50% positive for CD31 and this was only passage 3-4. Could you send another tube for me to test?

Answers:

Hi Jing

All of the cells provided by ScienCell are primary cells, not immortalized cell lines. It is highly recommended to use the primary cells at early passages without expanding and passaging the cells too much, especially for endothelial cells, since they are very sensitive and fragile. P3 or P4 is already considered as late passages for primary endothelial cells. It is not possible to get 100% pure population for primary cells. There may be ~2% fibroblasts in the endothelial cell culture. Endothelial cells may be damaged during each subculture if not handled with care, so fibroblasts would grow out eventually. (If we can have the lot number of #4000 that this customer received, I can send QC test result to him/her to show the purity of the cells). 

thanks for your interest

SCIENCELL TEAM