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人剪切应力和机械传导定量PCR芯片试剂盒
英文名:GeneQuery™ Human Shear Stress and Mechanotransduction qPCR Array Kit
货号:GK024
价格:¥4320.00
浏览量:1618次
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人剪切应力和机械传导定量PCR芯片试剂盒描述:


       ScienCell's GeneQuery™ Human Shear Stress and Mechanotransduction qPCR array kit (GQH-SSM) is designed to facilitate gene expression profiling of 88 key endothelial cell genes responsive to shear stress and mechanotransduction. Brief examples of how included genes may be grouped according to function are shown below:


       Signal transduction: CAV1, IP3R, MAPK1, PIAS1, SENP2

       ECM/cytoskeletal remodeling: COL4A2, DNM1, LAMA4, PMP22, VCAM1

       Immune response: IL13RA1, NFKB1, SELE, PTGS2, TLR2

       Transcription factors: GATA4, NCOR2, SP1, TRA2B, PPARG

       Ion transport: CCL14, NUP62, PIEZO1


       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.


       GeneQuery™ Human Shear Stress and Mechanotransduction qPCR Array Plate Layout* (8 controls in Bold and Italic)


 

1

2

3

4

5

6

7

8

9

10

11

12

A

A2M

CCL14

DUOX1

GATA3

KLF4

MFAP2

NUP62

PIK3CA

PTK2

SLC9A3R2

TAGLN

β-actin

B

ACTA2

CCL2

ECH1

GATA4

KRAS

MT1A

OLR1

PKD1

QDPR

SOD1

TLR2

GAPDH

C

ADAM15

CCNC

EDN3

GPC1

LAMA4

MYH10

P2RX1

PLAU

RHOA

SOD2

TOP1

LDHA

D

AKT1

CDK2

EFNA1

HMOX1

MAP1B

NCOR2

P2RX4

PPARA

SAE1

SP1

TRA2B

NONO

E

BBC3

COL4A2

EGR1

IL13RA1

MAPK1

NDRG1

P2RX5

PPARG

SELE

SRC

TYMS

PPIH

F

BDKRB2

CYBB

ELF2

ITPR3

MAPK7

NFKB1

PIAS1

PRKCE

SENP1

SREBF1

UBA2

GDC

G

BIRC2

CYC1

FABP1

JAK1

MDM2

NOX1

PIAS3

PTGIS

SENP5

SUMO2

UBE2I

PPC

H

CAV1

DNM1

GADD45A

JUN

MEF2A

NOX5

PIEZO1

PTGS2

SLC3A2

SUMO3

VCAM1

NTC


        * gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用

货号

GK024

产地

美国

缩写

GQH-SSM

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

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