人上皮细胞生物学定量PCR芯片试剂盒描述:
ScienCell's GeneQuery™ human epithelial cell biology qPCR array kit (GQH-EPI) is designed to facilitate gene expression profiling of key genes involved in human epithelial cell biology. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Epithelial Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
APC |
CDH2 |
DLL4 |
ERBB2 |
FZD5 |
HNF1A |
LGR5 |
NEUROG3 |
PDX1 |
ROCK2 |
STAT1 |
β-actin |
B |
APOC2 |
CDX1 |
EEF1B2 |
FGF7 |
FZD7 |
HNF1B |
MAX |
NKX2B |
PFDN2 |
RPL36 |
STK11 |
GAPDH |
C |
ATOH1 |
CDX2 |
EGFR |
FGFR1 |
FZD8 |
IHH |
MMP9 |
NOP58 |
PPARD |
SHROOM3 |
TARS |
LDHA |
D |
BLIMP1 |
CGN |
EIF2AK3 |
FGFR2 |
GATA4 |
JAM1 |
MSI1 |
NOTCH1 |
PTK6 |
SKIV2L |
TGFBR2 |
NONO |
E |
BMPR1A |
CLDN1 |
ELF3 |
FGFR3 |
GATA6 |
JAM2 |
MXD1 |
NOTCH2 |
RB1 |
SNAI1 |
TJP1 |
PPIH |
F |
CALB2 |
CLDN2 |
EPHB2 |
FKBP4 |
GFI1 |
KLF4 |
MYBL2 |
OCLN |
RBMS1 |
SOX9 |
VAV2 |
GDC |
G |
CBFA2T2 |
CTNNB1 |
EPHB3 |
FOXA1 |
GFM1 |
LDLR |
MYC |
PAX6 |
RBPJ |
SPDEF |
VIL2 |
PPC |
H |
CDH1 |
DDX56 |
EPRS |
FOXA2 |
GSN |
LGR4 |
NEUROD1 |
PCNA |
ROCK1 |
SPRR2A |
VIM |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
货号 |
GK005 |
产地 |
美国 |
缩写 |
GQH-EPI |
规格 |
One 96-well plate, one 20ul reaction per well |
用途 |
科研 |
保存 |
4°C or -20°C |
运输 |
常温 |
货号 | 产品名称 | 规格 | 价格 | 指令 |
2100 | 人表皮角质细胞--新生 | 5 x 10^5 cells/vial | ¥8370.00 | 放入购物车 》 |
2720 | 人食管上皮细胞 | 5x10^5cells/vial | ¥14616.00 | 放入购物车 》 |
3200 | 人肺泡上皮细胞 | 1 x 10^6 cells/vial | ¥15030.00 | 放入购物车 》 |
4100 | 人肾小管上皮细胞 | 5 x 10^5 cells/vial | ¥13932.00 | 放入购物车 》 |
6510 | 人角膜上皮细胞 | 5 x 10^5 cells/vial | ¥14040.00 | 放入购物车 》 |
7060 | 人宫颈上皮细胞 | 5 x 10^5 cells/vial | ¥14508.00 | 放入购物车 》 |
4101 | 上皮细胞培养基 | 500ML | ¥2256.00 | 放入购物车 》 |
2951 | 结肠上皮细胞培养基 | 500ML | ¥2112.00 | 放入购物车 》 |
3211 | 支气管上皮细胞培养基 | 500ML | ¥2256.00 | 放入购物车 》 |
6511 | 角膜上皮细胞培养基 | 500ML | ¥2160.00 | 放入购物车 》 |
2104 | 人表皮角质细胞cDNA | 20 reactions | ¥6552.00 | 放入购物车 》 |
2724 | 人食管上皮细胞cDNA | 20 reactions | ¥7087.00 | 放入购物车 》 |
4104 | 人肾近端小管上皮细胞cDNA | 20 reactions | ¥7087.00 | 放入购物车 》 |
6514 | 人角膜上皮细胞cDNA | 20 reactions | ¥7087.00 | 放入购物车 》 |
2105 | 人表皮角质细胞-新生儿总RNA | 10 μg | ¥5814.00 | 放入购物车 》 |
2725 | 人食管上皮细胞基因组总RNA | 10 μg | ¥6288.00 | 放入购物车 》 |
3205 | 人肺泡上皮细胞总RNA | 10 μg | ¥6288.00 | 放入购物车 》 |
4105 | 人肾小管上皮细胞总RNA | 10 μg | ¥6288.00 | 放入购物车 》 |
6515 | 人角膜上皮细胞总RNA | 10 μg | ¥6288.00 | 放入购物车 》 |