人成纤维生长因子信号通路定量PCR芯片试剂盒描述：

       ScienCell's GeneQuery™ Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit (GQH-FGF) is designed to facilitate gene expression profiling of 88 key genes involved in Fibroblast Growth Factor (FGF) signaling pathways. FGFs are involved in a wide variety of biological functions such as embryonic development, organogenesis, angiogenesis, tissue regeneration and wound healing. Through their binding with FGF receptors (FGFRs), FGFs activate and regulate many downstream signaling pathways, such as MAPK, JNK and STAT/NF-κB. Brief examples of how included genes may be grouped according to their functions are shown below:

       Secreted FGFs

       FGF1 superfamily: FGF1, FGF2

       FGF4 superfamily: FGF4, FGF5

       FGF7 superfamily: FGF3, FGF7, FGF10

       FGF8 superfamily: FGF17, FGF18

       FGF9 superfamily: FGF9, FGF16, FGF20

       Endocrine FGFs: FGF19, FGF23

       Intracellular FGFs: FGF13, FGF14

       FGF receptors: FGFR1, FGFR2, FGFR3, FGFR5

       FGF signaling inhibitors: IL17RD, SPRY2, SPRY4, PTPN1, DUSP1, DUSP6

       Activated downstream pathways

       MAPK pathway: FRS2, GAB1, SOS1, PTPN11, SHC1, RAS, BRAF, MAPK1, MAPK3, MAP2K1, MAP2K2

       PI3K/AKT pathway: AKT1, AKT2, AKT3, PI3Ks, FOXO1, FOXO3, BAD, BAX, CASP9

       JNK pathway: MAPK8, MAPK9, MAPK14, RAC1, ATF2, MAP2K4, MAP2K6

       STAT/NF-κB pathway: STAT1, STAT3, NFKB1, NFKB2, REL, MMP9

       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

       GeneQuery™ Human Fibroblast Growth Factor Signaling Pathway qPCR Array Plate Layout*(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development

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