人肝癌定量PCR芯片试剂盒描述：

       ScienCell's GeneQuery™ Human Hepatocellular Carcinoma qPCR Array Kit (GQH-HCC) is designed to facilitate gene expression profiling of 88 key genes involved in hepatocellular carcinoma tumorigenesis. Hepatocellular carcinoma is the most common type of liver cancer. It tends to occur in livers damaged by chronic infections of hepatitis B and/or C, alcohol abuse, type II diabetes and cirrhosis. Brief examples of how included genes may be grouped are shown below:

       Chronic hepatitis B virus and hepatitis C virus infection response: AKT1, NFKB1, SOCS3, TGFB1

       DNA damage response and apoptosis: CASP8, MSH2, BAX, BCL2, BIRC5, FAS, TP53

       Cell cycling: CCND1, CCND2, CDKN1A, CDKN2A, BIRC5, MYC, PTEN, RASSF1

       Major signaling pathways involved in HCC tumorigenesis

       Wnt/β-catenin: AXIN1, CCND1, CCND2, CTNNB1, EP300, FZD7, LEF1, RHOA, SFRP2, SMAD4

       VEGF: VEGFA, AKT1, KDR, PTGS2, PTK2, RAC1, NRAS

       FGF: AKT1, BAX, NFKB1, RAC1

       MAPK: EGF, FAS, GADD45B, PDGFRA, TGFB1, TGFBR2, RAC1

       PI3K: BCL2, FLT1, CREB3L3, IRS1, ITGB1, PTK1, MCL1, PTEN, RELN

       Other genes with demonstrated implications in HCC development: IGF2R, PDGFRL, MET, GLUL, MTUS1, FGL1, PEG10, MAGEC2, STARD13, FAH, GPC3

       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

       GeneQuery™ Human Hepatocellular Carcinoma qPCR Array Plate Layout*(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development

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