上海中乔新舟生物科技有限公司

ScienCell's GeneQuery™ Human Hepatitis B qPCR Array Kit (GQH-HPB) is designed to facilitate gene expression profiling of 88 key genes involved in hepatitis B pathogenesis. Hepatitis B is a liver infectious disease caused by the Hepatitis B virus (HBV). The HBV infection of hepatocytes triggers immune response cascades to regulate HBV replication and hepatocyte survival. Brief examples of how included genes may be grouped are shown below:

HBV binding factor: HSPG2, CPD

Signaling pathways

MAPK: MAPK1, MAP3K1, MAPK8, MAPK10, MAP2K1, RAF1, ELK1, YWHAB, YWHAQ, FOS

PI3K/AKT: AKT1, PIK3CA, PIK3CG, PIK3R1, PIK3R3, PIK3R5

Toll-like receptor: TLR2, TLR3, TLR4, IFNAs, IFNAR1, IFNB1

JAK/STAT: STAT1, STAT3, JAK1, MMP9, NFKB1, RELA, SRC

Genes implicated in HBV infection: LAMTOR5, RSF1, APOBEC3G, GPT, TNF, AIP, AFP, MBL2, F2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Hepatitis B qPCR Array Plate Layout*(8 controls in Bold and Italic)

AFP

ATP6AP1

DDX58

HSPG2

IFNA2

IFNB1

MAP2K4

MRC1

PTMA

SRC

TIRAP

β-actin

AIP

CPD

EGR3

IFIH1

IFNA21

IFNG

MAP3K1

NFKB1

RAF1

STAT1

TLR2

GAPDH

AKT1

CREB3

ELK1

IFNA1

IFNA4

IKBKE

MAPK1

PIK3CA

RARB

STAT3

TLR3

LDHA

ALB

CREB3L1

IFNA10

IFNA5

IRF3

MAPK10

PIK3CG

RELA

STAT6

TLR4

NONO

APOBEC3G

CREB3L2

FGL2

IFNA13

IFNA6

JAK1

MAPK8

PIK3R1

RFX1

TGFB1

TNF

PPIH

ASGR1

CTLA4

FOS

IFNA14

IFNA7

LAMTOR5

MAVS

PIK3R3

RSF1

TGFBR1

VDAC3

GDC

ATF4

DDB2

GGT1

IFNA16

IFNA8

MAP2K1

MBL2

PIK3R5

SLC17A5

TICAM1

YWHAB

PPC

ATF6B

DDX3X

GPT

IFNA17

IFNAR1

MAP2K2

MMP9

PSMA7

SON

TICAM2

YWHAQ

NTC

* gene selection may be updated based on new research and development

产品使用说明:仅供科研研究使用

货号	GK043
产地	美国
缩写	GQH-HPB
规格	One 96-well plate, one 20ul reaction per well
用途	科研
保存	4°C or -20°C
运输	常温

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