人肝星状细胞生物学定量PCR芯片试剂盒描述：

       ScienCell's GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Kit (GQH-HSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human hepatic stellate cell activation and proliferation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) hepatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:

       Hepatic stellate cell markers: ACTA2, GFAP, DES, NGFR, COL2A1, VIM, LAMA1, NES

       Hepatic stellate cell activation and proliferation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, RARA, PDGFs, TGFB1, MMP2

       Liver development and regeneration: STX2, PTN, SYNM, FOXF1, ICAM1, VCAM1, ITGB3, HGF, CXCL12, DES

       Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26

       ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF

       Cytokine production: EGF, IGFs, PDGFs, FGFs, CSF1, CCL2, TNF, CXCL2, CXCL3, CXCL8

       Hepatic fibrosis and cancer development: PKHD1, TMEM67, RPGRIP1L, SLC17A5, PKD1, CC2D2A, B9D1, B9D2

       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

       GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Plate Layout*(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development

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