上海中乔新舟生物科技有限公司

ScienCell's GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Kit (GQH-BRS) is designed to facilitate gene expression profiling of 88 key genes involved in B cell receptor signaling pathway. The B cell receptor signaling pathway is important for antibody production, B cell proliferation and differentiation. Brief examples of how included genes may be grouped according to their functions are shown below:

B cell receptor complex components: CD79A, CD79B

Co-inhibitory receptors: CD22, CD72, FCGR2B

Co-stimulators: CD81, CR2, IFITM1

Expression adaptor proteins: BLNK, GAB2, GRB2, CARD11

Kinases: LYN, SYK, PI3Ks

Phosphatases: INPP5D, PTPN6

MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3

PI3K/AKT pathway: PI3Ks, AKT1, AKT2

IKK/NFKB pathway: NFKBs, PRKCB, PLCG2, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Plate Layout*(8 controls in Bold and Italic)

AKT1

CD22

DAPP1

GSK3B

INPP5D

MAP2K1

MYC

NFKBIE

PIK3R3

PRKCD

RELA

β-actin

AKT2

CD72

ELK1

GTF2I

INPPL1

MAP3K7

NCK1

NRAS

PIP5K1A

PTPN18

SH3BP2

GAPDH

ATF2

CD79A

FCGR2B

HCLS1

IRF4

MAP4K1

NFATC1

PDPK1

PIP5K1C

PTPN6

SHC1

LDHA

BCL10

CD79B

FOS

HRAS

JUN

MAPK1

NFATC2

PIK3AP1

PLCG2

RAC1

SOS1

NONO

BLNK

CD81

FYN

IFITM1

KRAS

MAPK3

NFATC3

PIK3CA

PPP3CA

RAC2

SOS2

PPIH

BTK

CHUK

GAB2

IKBKB

LAT2

MAPK4

NFKB1

PIK3CB

PPP3CB

RAF1

SYK

GDC

CARD11

CR2

GRB2

IKBKG

LYN

MAPK9

NFKBIA

PIK3R1

PPP3R1

RASGRP3

TEC

PPC

CBL

DAG1

GSK3A

ILF2

MALT1

MEF2D

NFKBIB

PIK3R2

PRKCB

REL

VAV2

NTC

* gene selection may be updated based on new research and development

产品使用说明:仅供科研研究使用

货号	GK020
产地	美国
缩写	GQH-BRS
规格	One 96-well plate, one 20ul reaction per well
用途	科研
保存	4°C or -20°C
运输	常温

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