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人B细胞受体信号通路定量PCR芯片试剂盒

英文名:GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Kit
货号:GK020
价格:¥4320.00
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人B细胞受体信号通路定量PCR芯片试剂盒描述:


       ScienCell's GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Kit (GQH-BRS) is designed to facilitate gene expression profiling of 88 key genes involved in B cell receptor signaling pathway. The B cell receptor signaling pathway is important for antibody production, B cell proliferation and differentiation. Brief examples of how included genes may be grouped according to their functions are shown below:


       B cell receptor complex components: CD79A, CD79B

       Co-inhibitory receptors: CD22, CD72, FCGR2B

       Co-stimulators: CD81, CR2, IFITM1

       Expression adaptor proteins: BLNK, GAB2, GRB2, CARD11

       Kinases: LYN, SYK, PI3Ks

       Phosphatases: INPP5D, PTPN6

       MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3

       PI3K/AKT pathway: PI3Ks, AKT1, AKT2

       IKK/NFKB pathway: NFKBs, PRKCB, PLCG2, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG


       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.


       GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Plate Layout*(8 controls in Bold and Italic)


 

1

2

3

4

5

6

7

8

9

10

11

12

A

AKT1

CD22

DAPP1

GSK3B

INPP5D

MAP2K1

MYC

NFKBIE

PIK3R3

PRKCD

RELA

β-actin

B

AKT2

CD72

ELK1

GTF2I

INPPL1

MAP3K7

NCK1

NRAS

PIP5K1A

PTPN18

SH3BP2

GAPDH

C

ATF2

CD79A

FCGR2B

HCLS1

IRF4

MAP4K1

NFATC1

PDPK1

PIP5K1C

PTPN6

SHC1

LDHA

D

BCL10

CD79B

FOS

HRAS

JUN

MAPK1

NFATC2

PIK3AP1

PLCG2

RAC1

SOS1

NONO

E

BLNK

CD81

FYN

IFITM1

KRAS

MAPK3

NFATC3

PIK3CA

PPP3CA

RAC2

SOS2

PPIH

F

BTK

CHUK

GAB2

IKBKB

LAT2

MAPK4

NFKB1

PIK3CB

PPP3CB

RAF1

SYK

GDC

G

CARD11

CR2

GRB2

IKBKG

LYN

MAPK9

NFKBIA

PIK3R1

PPP3R1

RASGRP3

TEC

PPC

H

CBL

DAG1

GSK3A

ILF2

MALT1

MEF2D

NFKBIB

PIK3R2

PRKCB

REL

VAV2

NTC

 

* gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用

货号

GK020

产地

美国

缩写

GQH-BRS

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

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