人非小细胞肺癌定量PCR芯片试剂盒描述:
ScienCell's GeneQuery™ Human Non-Small Cell Lung Cancer qPCR Array Kit (GQH-NLC) is designed to facilitate gene expression profiling of 88 key genes involved in non-small cell lung cancer (NSCLC) carcinogenesis. NSCLC accounts for more than 80% of all lung cancers. There are three main types of NSCLC: adenocarcinoma, lung squamous cell carcinoma and lung large cell carcinoma. Brief examples of how included genes may be grouped according to their functions are shown below:
Oncogenes and tumor suppressor genes: KRAS, HRAS, EML4, ALK, RARB, FHIT, RASSF1, TP53, CDKN2A
Cell cycle regulation and apoptosis: CDK4, CDK6, CCND1, CCNE2, CASP3, CASP9, BAD, STK4, FOXO3
MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
PI3K/AKT pathway: PI3Ks, AKT1, AKT2
Genes related to specific lung cancer types
Adenocarcinoma: BRAF, ERBB2, TGFBR2, SMAD4, EPCAM, BIRC5
Lung squamous cell carcinoma: FGFR2, CYPIA1, PTHLH, SOX2, PEBP1
Lung large cell carcinoma: ROS1, NME1, TTN, MYO18B, EPHA5, WNK3
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Non-Small Cell Lung Cancer qPCR Array Plate Layout*(8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
ACTR3B |
CALB2 |
CDK4 |
EPHA3 |
GRAMD4 |
MAP2K2 |
NRAS |
PIK3R1 |
RAF1 |
RXRA |
SOX2 |
β-actin |
B |
AKT1 |
CASK |
CDK6 |
EPHA5 |
GRB2 |
MAPK1 |
NTRK3 |
PIK3R3 |
RAP2B |
RXRG |
STK11 |
GAPDH |
C |
AKT2 |
CASP3 |
CDKN2A |
ERBB2 |
HRAS |
MAPK3 |
OGG1 |
PIK3R5 |
RARB |
S100A2 |
STK4 |
LDHA |
D |
ALK |
CASP9 |
CYP1A1 |
FAM83B |
KRAS |
MMP2 |
PDPK1 |
PLCG2 |
RASSF1 |
S100A7 |
TGFA |
NONO |
E |
ARAF |
CCND1 |
DDR2 |
FGFR1 |
KRT19 |
MUC1 |
PEBP1 |
PRKCG |
RASSF5 |
SFTPB |
TP53 |
PPIH |
F |
BAD |
CDC42BPB |
E2F2 |
FHIT |
KRT20 |
MYO18B |
PIK3CA |
PRKCI |
RB1 |
SFTPC |
TTN |
GDC |
G |
BRAF |
CDH1 |
EGF |
FIGF |
KRT7 |
NKX2-1 |
PIK3CD |
PTGS2 |
RBMS3 |
SOS1 |
VEGFA |
PPC |
H |
CADM1 |
CDH10 |
EML4 |
FOXO3 |
MAP2K1 |
NME1 |
PIK3CG |
PTHLH |
ROS1 |
SOS2 |
WNK3 |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
货号 |
GK033 |
产地 |
美国 |
缩写 |
GQH-NLC |
规格 |
One 96-well plate, one 20ul reaction per well |
用途 |
科研 |
保存 |
4°C or -20°C |
运输 |
常温 |
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