上海中乔新舟生物科技有限公司

ScienCell's GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation. HIF1 activity is a major component of the human response to hypoxia. Brief examples of how included HIF1 downstream genes may be grouped according to regulatory function are shown below:

Cell cycling: DHFR, MAP3K5, POLE2, PRC1, SKP2

Extracellular matrix remodeling: COL1A1, COL5A1, LOX, P4HA1, PLOD1

Angiogenesis: HMOX1, MMP2, VEGFA, ANGPTL4, PGF

Metabolism: PKM, STC2, DHX35, DDX11, ERO1A

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ Human HIF1 Signaling Response qPCR Array Plate Layout* (8 controls in Bold and Italic)

ABCB1

BHLHE40

COL18A1

DUSP6

HMOX1

LOXL2

NEK4

PKM

PRC1

RRAS

TSFM

β-actin

ADORA2A

BRCA1

COL1A1

ELK1

IGFBP3

MAP3K5

NOTCH4

PLCG2

PSME3

RRM2

TXNIP

GAPDH

ADORA2B

CCNE1

COL1A2

ERO1A

INHBA

MCM4

P4HA1

PLOD1

PTGS1

SKP2

UBE2S

LDHA

AK3

CCNE2

COL5A1

EXOSC2

INHBE

MEF2A

P4HA2

PLOD2

PTPRB

SMAD9

VEGFA

NONO

ANGPTL4

CCNF

CXCR4

FOS

INSR

MLLT10

PAM

POLE2

PTPRR

STC1

VEGFC

PPIH

BARD1

CDC6

DDX11

GADD45B

KDM3A

MMP2

PDZD2

POLR3K

RB1

STC2

VLDLR

GDC

BCL2

CDK1

DHFR

GINS2

LEPR

MXI1

PELI2

PPARD

RFC5

TFAM

ZNF292

PPC

BCL6

CDKN1C

DHX35

HES1

LOX

NDRG1

PGF

PPARG

RNH1

TRIP13

ZWINT

NTC

*gene selection may be updated based on new research and development

产品使用说明:仅供科研研究使用

货号	GK036
产地	美国
缩写	GQH-HFT
规格	One 96-well plate, one 20ul reaction per well
用途	科研
保存	4°C or -20°C
运输	常温

1.)Atorvastatin inhibits the HIF1α-PPAR axis, which is essential for maintaining the function of human induced pluripotent stem cells,Mol Ther. 2018 Jul 5; 26(7): 1715–1734.Published online 2018 Jun 19. doi: 10.1016/j.ymthe.2018.06.005

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