上海中乔新舟生物科技有限公司

ScienCell's GeneQuery™ Human Endothelial Cell Heterogeneity and Differentiation qPCR Array Kit (GQH-EHD) is designed to facilitate gene expression profiling of 88 key genes to help characterize endothelial cells during lineage differentiation and is based on extensive literature research. Brief examples of how included genes may be grouped according to specification markers are shown below:

Circulating Early Endothelial Progenitors: MAFB, RB1, ADORA3, MYCL1, NCOA2

Circulating Late Endothelial Progenitors: FOXF1, ITGAM, SIX4, EGFR, CREB5

Hemangioblasts: GATA1, LMO2, PROM1, RUNX1, TAL1

Lymphatic Endothelial Cells: FLT4, LYVE1, PDPN, PROX1, FGFR3

EndMT: ACTA2, SMAD3, S100A4, TWIST, BMP7

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ Human Astrocyte Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

ANGPT1

CDH2

DVL3

EREG

FOXO1

IL1B

KIT

MMP9

PDGFRA

SELE

TGFB1

β-actin

ANGPT2

CDH5

EDN1

FGF1

FZD4

IL6

KLF2

NOS3

PDGFRB

SEMA3F

TGFB2

GAPDH

BAIAP3

CNTN1

EDNRA

FGF2

HAND2

ITGA5

KLK3

NOTCH1

PECAM1

SERPINE1

THBS1

LDHA

BMP1

COL4A3

EFNB2

FIGF

HEY2

ITGAV

LAMA5

NOTCH4

PF4

SERPINF1

TNFAIP1

NONO

CCL2

CX3CL1

EGLN1

FLT1

HHEX

ITGB1

LGALS9

NPPB

PLAU

SMAD2

TYMP

PPIH

CCL5

CXCR4

ENG

FN1

HIF1A

ITGB3

MAGI1

NRP1

PODXL

SMAD3

VCAM1

GDC

CD36

DICER1

EPHA2

FOS

ICAM1

JAG1

MEOX2

NRP2

PTGS2

SPHK1

VEGFA

PPC

CD82

DLL4

ERBB2

FOXC2

ID1

KDR

MMP2

PARD3

RUNX2

TEK

VEGFC

NTC

* gene selection may be updated based on new research and development

产品使用说明:仅供科研研究使用

货号	GK023
产地	美国
缩写	GQH-ECD
规格	One 96-well plate, one 20ul reaction per well
用途	科研
保存	4°C or -20°C
运输	常温

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