人心血管疾病定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Cardiovascular Disease qPCR Array Kit (GQH-CVD) is designed to facilitate gene expression profiling of 88 key genes involved in the pathogenesis of heart and coronary artery diseases. Genes are included but not limited to such as atherosclerosis, myocardial infarction, and ischemic stroke. Brief examples of how included genes may be grouped according to pathology are shown below:
Risk factors: APOB, ACE, SELE, SERPINE1, PPARA
Myocardial infarction: OLR1, LRP8, LGALS2, ITGB3, LTA, F7
Arteriosclerosis: APOA1, PON1, ADIPOQ, MGP, ICAM1
Atherosclerosis: CCL2, ABCG8, PPARG, LDLR, APOC2
Ischemic stroke: NOS3, PTGIS, PLAT, MTHFR
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Cardiovascular Disease qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ABCA1 |
AGTR1 |
CBS |
CSF1 |
F7 |
HSPD1 |
LIPC |
MGP |
OLR1 |
PON3 |
SELP |
β-actin |
|
B |
ABCG5 |
ALOX5 |
CCL2 |
CXCR3 |
FGF1 |
ICAM1 |
LPA |
MMP3 |
PDGFB |
PPARA |
SERPINE1 |
GAPDH |
|
C |
ABCG8 |
APOA1 |
CCT7 |
DAB2IP |
GCLC |
IL6 |
LPL |
MMP9 |
PF4 |
PPARD |
SPP1 |
LDHA |
|
D |
ACE |
APOA2 |
CD40 |
EDN1 |
GCLM |
ITGA2 |
LRP6 |
MSR1 |
PIK3C2A |
PPARG |
TGFB1 |
NONO |
|
E |
ADIPOQ |
APOA5 |
CEL |
ELN |
GJA4 |
ITGA2B |
LRP8 |
MTHFR |
PLA2G7 |
PSMA6 |
THBD |
PPIH |
|
F |
ADRB1 |
APOB |
CETP |
ESR1 |
GUCY1A3 |
ITGB3 |
LTA |
NOS3 |
PLAT |
PTGIS |
TNFSF4 |
GDC |
|
G |
AGER |
APOC2 |
COG2 |
F13A1 |
HGF |
LDLR |
MB |
NPPA |
PON1 |
SCARB1 |
VCAM1 |
PPC |
|
H |
AGT |
APOE |
CRP |
F3 |
HMOX1 |
LEP |
MEF2A |
NPPB |
PON2 |
SELE |
VWF |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK055 |
|
产地 |
美国 |
|
缩写 |
GQH-CVD |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 |
上海中乔新舟生物科技有限公司
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