小鼠IL-6ELISA试剂盒描述:
The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa. ScienCell's mouse IL-6 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse IL-6-specific monoclonal antibodies are precoated onto 8 x 12 strips. The mouse specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse IL-6 in the sample that is captured in the strips.
产品使用说明:仅供科研研究使用
货号 |
EK0411 |
产地 |
美国 |
缩写 |
m IL-6-ELISA |
规格 |
96 tests |
用途 |
科研 |
保存 |
频繁使用存储在4°C,很少使用存储在-20°C |
运输 |
胶冰 |
参考文献 |
1. Bowcock, A. M.; Kidd, J. R.; Lathrop, G.
M.; Daneshvar, L.; May, L. T.; Ray, A.; Sehgal, P. B.; Kidd, K. K.;
Cavalli-Sforza, L. L. The human 'interferon-beta-2/hepatocyte stimulating
factor/interleukin-6' gene: DNA polymorphism studies and localization to
chromosome 7p21. Genomics 3: 8-16, 1988. |
2. Ota, N.; Nakajima, T.; Nakazawa, I.; Suzuki, T.; Hosoi, T.; Orimo, H.; Inoue, S.; Shirai, Y.; Emi, M. A nucleotide variant in the promoter region of the interleukin-6 gene associated with decreased bone mineral density. J. Hum. Genet. 46: 267-272, 2001.
3. Chung, H. W.; Seo, J.-S.; Hur, S. E.; Kim, H. L.; Kim, J. Y.; Jung, J. H.; Kim, L. H.; Park, B. L.; Shin, H. D. Association of interleukin-6 promoter variant with bone mineral density in pre-menopausal women. J. Hum. Genet. 48: 243-248, 2003.
4. Ferrari, S. L.; Ahn-Luong, L.; Garnero, P.; Humphries, S. E.; Greenspan, S. L. Two promoter polymorphisms regulating interleukin-6 gene expression are associated with circulating levels of C-reactive protein and markers of bone resorption in postmenopausal women. J. Clin. Endocr. Metab. 88: 255-259, 2003.
5. Kiecolt-Glaser, J. K.; Preacher, K. J.; MacCullum, R. C.; Atkinson, C.; Malarkey, W. B.; Glaser, R. Chronic stress and age-related increases in the proinflammatory cytokine IL-6. Proc. Nat. Acad. Sci. 100: 9090-9095, 2003.
6. Hirano, T.; Yasukawa, K.; Harada, H.; Taga, T.; Watanabe, Y.; Matsuda, T.; Kashiwamura, S.; Nakajima, K.; Koyama, K.; Iwamatsu, A.; Tsunasawa, S.; Sakiyama, F.; Matsui, H.; Takahara, Y.; Taniguchi, T.; Kishimoto, T. Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin. Nature 324: 73-76, 1986.
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