大鼠IL-1αELISA试剂盒描述:
Interleukin-1α (IL-1α) and interleukin-1β (IL-1β) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1α has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1α is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1α gene is assigned to chromosome 2. Genetic polymorphisms at IL-1α and IL-1β have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1α and IL-1β genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1α with the molecular mass of 18kDa. ScienCell's rat IL-1α ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat IL-1α specific-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The rat specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat IL-1α captured in strips.
产品使用说明:仅供科研研究使用
货号 |
EK0390 |
产地 |
美国 |
缩写 |
rIL-1α-ELISA |
规格 |
96 tests |
用途 |
科研 |
保存 |
频繁使用存储在4°C,很少使用存储在-20°C |
运输 |
胶冰 |
参考文献 |
1. Lafage, M.; Maroc, N.; Dubreuil, P.; de
Waal Malefijt, R.; Pebusque, M.-J.; Carcassonne, Y.; Mannoni, P. The human
interleukin-1-alpha gene is located on the long arm of chromosome 2 at band
q13. Blood 73: 104-107, 1989. |
2. Bailly, S.; di Giovine, F. S.; Blakemore, A. I. F.; Duff, G. W. Genetic polymorphism of human interleukin-1-alpha. Europ. J. Immun. 23: 1240-1245, 1993.
3. Modi, W. S.; Masuda, A.; Yamada, M.; Oppenheim, J. J.; Matsushima, K.; O'Brien, S. J. Chromosomal localization of the human interleukin 1a (IL-1-alpha) gene. Genomics 2: 310-314, 1988.
4. Diehl, S. R.; Wang, Y.; Brooks, C. N.; Burmeister, J. A.; Califano, J. V.; Wang, S.; Schenkein, H. A. Linkage disequilibrium of interleukin-1 genetic polymorphisms with early-onset periodontitis. J. Periodont. 70: 418-430, 1999.
5. Silver, A. R. J.; Masson, W. K.; George, A. M.; Adam, J.; Cox, R. The Il-1 alpha and beta genes are closely linked (less than 70 kb) on mouse chromosome 2. Somat. Cell Molec. Genet. 16: 549-556, 1990.
货号 | 产品名称 | 规格 | 价格 | 指令 |
EK0326 | 小鼠表皮生长因子ELISA试剂盒 | 96 tests | ¥6660.00 | 放入购物车 》 |
EK0333 | 小鼠促红细胞生成素ELISA试剂盒 | 96 tests | ¥7560.00 | 放入购物车 》 |
EK0351 | 小鼠胚胎纤维连接蛋白ELISA试剂盒 | 96 tests | ¥4365.00 | 放入购物车 》 |
EK0371 | 小鼠细胞间粘附分子ELISA试剂盒 | 96 tests | ¥7020.00 | 放入购物车 》 |
EK0403 | 小鼠IL-3ELISA试剂盒 | 96 tests | ¥6660.00 | 放入购物车 》 |
EK0411 | 小鼠IL-6ELISA试剂盒 | 96 tests | ¥5580.00 | 放入购物车 》 |