您是第 1594570 位 欢迎访问 中乔新舟官网 ! 全国免费电话:400-038-9959 我的购物车(0) 注 册 / 登 录
您当前的位置:首页 > 产品中心
大鼠血管内皮细胞生长因子ELISA试剂盒
英文名:Rat VEGF ELISA Kit
货号:EK0540
价格:¥5580.00
浏览量:1652次
加入购物车,提交订单信息之后,我们会第一时间与您取得联系!
  • 产品说明
  • 产品规格
  • 参考文献
  • 常见问题

大鼠血管内皮细胞生长因子ELISA试剂盒描述:


       Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis, and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant rat VEGF164, which is a 25 kDa single chain, as well as a 50 kDa dimer. ScienCell's rat VEGF ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat VEGF-specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The rat-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat VEGF in the sample that is captured on the strips.


产品使用说明:仅供科研研究使用

货号

EK0540

产地

美国

缩写

rVEGF-ELISA

规格

96 tests

用途

科研

保存

频繁使用存储在4°C,很少使用存储在-20°C

运输

胶冰

参考文献

1. Basu, S.; Nagy, J. A.; Pal, S.; Vasile, E.; Eckelhoefer, I. A.; Bliss, V. S.; Manseau, E. J.; Dasgupta, P. S.; Dvorak, H. F.; Mukhopadhyay, D. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability Factor/vascular endothelial growthFactor. Nature Med. 7: 569-574, 2001. 
2. Poulaki, V.; Mitsiades, C. S.; McMullan, C.; Sykoutri, D.;Fanourakis, G.; Kotoula, V.; Tseleni-Balafouta, S.; Koutras, D. A.; Mitsiades, N. Regulation of vascular endothelial growthFactor expression by insulin-like growthFactor I in thyroid carcinomas. J. Clin. Endocr. Metab. 88: 5392-5398, 2003.
 
3. Awata, T.; Inoue, K.; Kurihara, S.; Ohkubo, T.; Watanabe, M.; Inukai, K.; Inoue, I.; Katayama, S. A common polymorphism in the 5-prime-untranslated region of the VEGF gene is associated with diabetic retinopathy in type 2 diabetes. Diabetes 51: 1635-1639, 2002.
 
4. Soker, S.; Takashima, S.; Miao, H. Q.; Neufeld, G.; Klagsbrun, M. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptorFor vascular endothelial growthFactor. Cell 92: 735-745, 1998.
 
5. Wei, M.-H.; Popescu, N. C.; Lerman, M. I.; Merrill, M. J.; Zimonjic, D. B. Localization of the human vascular endothelial growthFactor gene, VEGF, at chromosome 6p12. Hum. Genet. 97: 794-797, 1996.

       1. Basu, S.; Nagy, J. A.; Pal, S.; Vasile, E.; Eckelhoefer, I. A.; Bliss, V. S.; Manseau, E. J.; Dasgupta, P. S.; Dvorak, H. F.; Mukhopadhyay, D. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability Factor/vascular endothelial growthFactor. Nature Med. 7: 569-574, 2001. 


       2. Poulaki, V.; Mitsiades, C. S.; McMullan, C.; Sykoutri, D.;Fanourakis, G.; Kotoula, V.; Tseleni-Balafouta, S.; Koutras, D. A.; Mitsiades, N. Regulation of vascular endothelial growthFactor expression by insulin-like growthFactor I in thyroid carcinomas. J. Clin. Endocr. Metab. 88: 5392-5398, 2003. 


       3. Awata, T.; Inoue, K.; Kurihara, S.; Ohkubo, T.; Watanabe, M.; Inukai, K.; Inoue, I.; Katayama, S. A common polymorphism in the 5-prime-untranslated region of the VEGF gene is associated with diabetic retinopathy in type 2 diabetes. Diabetes 51: 1635-1639, 2002. 


       4. Soker, S.; Takashima, S.; Miao, H. Q.; Neufeld, G.; Klagsbrun, M. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptorFor vascular endothelial growthFactor. Cell 92: 735-745, 1998. 


       5. Wei, M.-H.; Popescu, N. C.; Lerman, M. I.; Merrill, M. J.; Zimonjic, D. B. Localization of the human vascular endothelial growthFactor gene, VEGF, at chromosome 6p12. Hum. Genet. 97: 794-797, 1996.

There are no product questions yet.
公司简介 / Company profile
上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
       公司主要依托复旦大学、同济大学等上海地区多家著名高校,拥有一支富有经验的开发团队,专业从事细胞及细胞...
联系我们 / Contact
电 话:021-56760357;021-56145703;021-56468627
传 真:021-56760351
邮 箱:sales@zqxzbio.com
邮 编:200439
地 址:上海市宝山区长江南路180号A区403-406室
Copyright © 2014 ZQXZBIO All rights reserved.

沪公网安备 31011002001038号

技术支持:攸攸网络 沪ICP备14008091号
在线咨询
免费热线
400-038-9959

QQ咨询
我们竭诚为您服务!