人心脏成纤维细胞说明：
心脏成纤维细胞（CF）是构成心脏的主要细胞类型。它们对于心肌细胞起结构支持作用，在生长和病理条件下，负责细胞外基质的合成。CF是心肌损伤反应的重要细胞成分，并能产生旁分泌生长因子。CF的增殖和基质合成对于心肌梗塞、心肌纤维化部位瘢痕形成具有重要作用，并常并发心脏肥厚。CF通过调节整合素的表达来适应病理情况下周围环境的变化。CF的培养已被广泛应用为生理（运动）和病理（高血压）应激下心脏基质重塑研究的模型。
人心脏成纤维细胞(HCF)提取于人心脏组织，于原代冻存。每管含有细胞数>5×105cells/ml。此细胞通过纤维连接蛋白抗体免疫荧光检测方法验证。经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。
推荐培养基:成纤维细胞专用培养液 (FM, Cat. No. 2331)
产品使用说明：仅供科研研究使用
1.) Chida J, Araki H, Maeda Y. (2014) "Specific growth suppression of human cancer cells by targeted delivery of Dictyostelium mitochondrial ribosomal protein S4." Cancer cell int. 14: 56.
2.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC Jr. (2010) "B-type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J Cell Physiol. 225: 251-5.
3.) Lin CS, Pan CH, Wen CH, Yang TH, Kuan TC. (2010) "Regulation of angiotensin converting enzyme II by angiotensin peptides in human cardiofibroblasts." Peptides. 31: 1334-40.
4.) Westenbrink BD, Ruifrok WP, Voors AA, Tilton RG, van Veldhuisen DJ, Schoemaker RG, van Gilst WH, de Boer RA. (2010) "Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure." Cardiovasc Res. 87: 30-9.
5.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) "B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J of cell physiol. 225: 251-55.
6.) McKie PM, Cataliotti A, Huntley BK, Martin FL, Olson TM, Burnett JC Jr. (2009) "A human atrial natriuretic peptide gene mutation reveals a novel peptide with enhanced blood pressure-lowering, renal-enhancing, and aldosterone-suppressing actions." J Am Coll Cardiol. 54: 1024-32.
7.) Lisy O, Huntley BK, McCormick DJ, Kurlansky PA, Burnett JC Jr. (2008) "Design, synthesis, and actions of a novel chimeric natriuretic peptide: CD-NP." J Am Coll Cardiol. 52: 60-8.
8.) Pan CH, Wen CH, Lin CS. (2008) "Interplay of angiotensin II and angiotensin(1-7) in the regulation of matrix metalloproteinases of human cardiocytes." Exp Physiol. 93: 599-612.
9.) Reddy VS, Harskamp RE, van Ginkel MW, Calhoon J, Baisden CE, Kim IS, Valente AJ, Chandrasekar B. (2008) "Interleukin-18 stimulates fibronectin expression in primary human cardiac fibroblasts via PI3K-Akt-dependent NF-kappaB activation." J Cell Physiol. 215: 697-707.
10.) Saxena A, Fish JE, White MD, Yu S, Smyth JW, Shaw RM, DiMaio JM, Srivastava D. (2008) "Stromal cell-derived factor-1alpha is cardioprotective after myocardial infarction." Circulation. 117: 2224-31.
11.) Bunda S, Liu P, Wang Y, Liu K, Hinek A. (2007) "Aldosterone induces elastin production in cardiac fibroblasts through activation of insulin-like growth factor-I receptors in a mineralocorticoid receptor-independent manner." Am J Pathol. 171: 809-19.
12.) Cortez DM, Feldman MD, Mummidi S, Valente AJ, Steffensen B, Vincenti M, Barnes JL, Chandrasekar B. (2007) "IL-17 stimulates MMP-1 expression in primary human cardiac fibroblasts via p38 MAPK- and ERK1/2-dependent C/EBP-beta , NF-kappaB, and AP-1 activation." Am J Physiol Heart Circ Physiol. 293: H3356-65.
13.) Noser JA, Mael AA, Sakuma R, Ohmine S, Marcato P, Lee PW, Ikeda Y. (2007) "The RAS/Raf1/MEK/ERK Signaling Pathway Facilitates VSV-mediated Oncolysis: Impli cation for the Defective Interferon Response in Cancer Cells." Mol Ther. 15: 1531-6.
14.) Huntley BK, Sandberg SM, Noser JA, Cataliotti A, Redfield MM, Matsuda Y, Burnett JC Jr. (2006) "BNP-induced activation of cGMP in human cardiac fibroblasts: Interactions with fibronectin and natriuretic peptide receptors." J Cell Physiol. 209: 943-9.
15.)Doxorubicin induces trans-differentiation and MMP1 expression in cardiac fibroblasts via cell death-independent pathways,PLoS One. 2019; 14(9): e0221940.Published online 2019 Sep 12. doi: 10.1371/journal.pone.0221940
16.)Tumor‐stroma interactions differentially alter drug sensitivity based on the origin of stromal cells,Mol Syst Biol. 2018 Aug; 14(8): e8322.Published online 2018 Aug 6. doi: 10.15252/msb.20188322
上海中乔新舟生物科技有限公司
微信扫一扫咨询
