人视网膜是由位于眼睛后部多层结构组成的。它包括视细胞和视网膜色素上皮细胞（RPE），视网膜色素细胞通过提供营养和代谢废物来滋养视网膜。RPE位于神经视网膜和脉络膜之间，成为血视网膜屏障的外层控制着视网膜下间隙的化学成分，人们发现RPE在许多蛋白质的极性方面不同于其他上皮细胞。几乎所有的上皮细胞的顶生面都没有基质，而RPE的顶生面胞膜是与细胞外基质相接触的。在早期胚胎发育过程中，RPE自发转分化为神经视网膜组织，RPE在其邻近的光感受器的发育和维持中有着重要的作用，光照条件下RPE能产生大量的活性氧，RPE的增殖也是许多眼病发病过程中重要的一步，如增殖性视网膜病变。   

      人视网膜色素上皮细胞(HRPEpiC)提取于人视网膜组织，于原代冻存。每管含有细胞数>5×105cells/ml，此细胞通过Cytokeratin-18, Cytokeratin-19 and Fibronectin免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到10倍增。

推荐培养基（EpiCM, Cat No 4101）

产品使用说明：仅供科研研究使用

8.) Hunt DW, Boivin WA, Fairley LA, Jovanovic MM, King DE, Salmon RA, Utting OB. (2006) "Ultraviolet B light stimulates interleukin-20 expression by human epithelial keratinocytes." Photochem Photobiol. 82: 1292-300.

9.)The polyether ionophore salinomycin targets multiple cellular pathways to block proliferative vitreoretinopathy pathology,PLoS One. 2019; 14(9): e0222596.Published online 2019 Sep 17. doi: 10.1371/journal.pone.0222596

Question:

Regarding Human Retinal Pigment Epithelial Cells Catalog #6540 Are the cells from a single human donor or multiple in a pool? If single donor, can one order vials from a few donors to compare? Can you provide a list of available donors and their characteristics? I have read the following paper where this product is used and they make the comment that they conducted their work with hRPEpC in multiple donors. Toxicol Sci. 2015 Mar;144(1):163-72. Retinal Toxicity, in vivo and in vitro, Associated with Inhibition of Nicotinamide Phosphoribosyltransferase.

Answers:

• Posted by Manoj Sharma on Thursday, January 7, 2016

  Hi Elizabeth,

  it is Single donor.if you are interested in multiple vials from different lots to get different donors. Please contact us at techsupport@sciencellonline.com to obtain donor information.
  Thanks for your interest in our products.

  SCIENCELL TEAM