产品名称 |
MV4-11人急性单核细胞白血病细胞 |
货号 |
ZQ0464 |
产品介绍 |
MV-4-11细胞系是从患有人双表型髓性单核细胞白血病(biphenotypic B myelomonocytic leukemia)的10岁男孩的外周血。Interleukin (IL)-3可以独立地支持该细胞长期生长,使用10%FBS培养时则不需要额外添加IL-3。但是,在IL-3和生长因子Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF)均处于低浓度的情况下,IL-3会抑制MV-4-11细胞的增殖。生长因子Granulocyte Colony Stimulating Factor(G-CSF)会协同GM-CSF促进MV-4-11细胞的增殖,而单独的G-CSF会短暂刺激该细胞系。据文献[PubMed: 3500218]报道,间接免疫荧光法检测髓性单核细胞抗原CD15,超过96%的该细胞为阳性,40~96%为单核细胞抗原CD4阳性,4-11%为单核细胞抗原CD10阳性。
MV4-11细胞的特点是其高增殖率和存在某些遗传异常。4号和11号染色体之间的易位导致MLL-AF4融合基因的产生,该基因在白血病发生中起着至关重要的作用,并有助于白血病的侵袭性。MLL-AF4融合基因的存在使得这些细胞与理解白血病发生的分子机制以及旨在破坏这种致癌融合蛋白功能的靶向治疗研究特别相关。此外,该细胞可用于研究白血病干细胞的生物学、耐药机制以及骨髓微环境在白血病进展中的作用。该细胞系进一步有助于代谢组学和转录组学研究,为白血病的代谢改变和氧化还原适应提供全面的了解。MV-4-11细胞对各种癌症研究化学物质(包括venetoclax等抑制剂)的反应能力,以及它们在研究耐药细胞中的作用。总之该细胞系是白血病研究的重要工具,为研究急性髓系白血病的复杂生物学、测试治疗药物的疗效以及探索靶向治疗克服耐药性的潜力提供了一个多功能平台。
【注意事项】: |
种属 |
人 |
性别/年龄 |
男/10岁 |
组织 |
外周血 |
疾病 |
双表型B粒单核细胞白血病 |
细胞类型 |
巨噬细胞 |
形态学 |
成淋巴细胞 |
生长方式 |
悬浮 |
倍增时间 |
大约32-50小时 |
培养基和添加剂 |
IMDM(品牌:中乔新舟 货号:ZQ-900)+15%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13 D16S539: 11,12 D5S818: 11,12 D7S820: 8,9 THO1: 8,9.3 TPOX: 8,11 vWA: 14,15 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-9591 DSMZ; ACC-102 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0464 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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