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人脐静脉内皮细胞

英文名:Human Umbilical Vein Endothelial Cells
货号:8000
价格:¥5148.00
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人脐静脉内皮细胞说明:

    

         血管内皮细胞对维持血管动态平衡起着重要作用。它们合成、分泌凝血和纤溶系统的激活因子和抑制因子、影响血小板粘附和聚集的调节因子。内皮细胞还释放控制细胞增殖和调节血管壁紧张度的分子。这些过程都可以用培养的细胞进行体外研究,人脐静脉和动脉内皮细胞是这类研究中常用的细胞类型。除了普通的内皮细胞特征例如“鹅卵石状”,VIII因子阳性着色,能摄取乙酰基化低密度脂蛋白,人脐血管内皮细CD31染色阳性。用IL-1或TNF-α预先处理过的细胞也能选择性地表达E-selectin和VCAM。    

       

        人脐静脉内皮细胞(HUVEC)提取于人脐带静脉组织,原代冻存。每管含有细胞数>5×105 cells/ml,此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。


配套培养基:内皮细胞培养基(Cat.No.1001)


产品使用说明:仅供科研研究使用




货号 8000
产地 美国
缩写 HUVEC
规格 5 x 10^5 cells/vial
用途 科研
运输 干冰
保存 液氮
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13.) Yang, G., Li, Y., Wu, W., Liu, B., Ni, L., Wang, Z., Miao, S., Wang, L. & Liu, C. (2015) Anti-oxidant effect of heme oxygenase-1 on cigarette smoke-induced vascular injuryMol Med Rep. 12

14.) Ye, Q., Qin, S., Liu, Y., Feng, J., Wu, Q., Qu, W. & Yin, X. (2015) Inhibitory Effect of Endostar on Specific Angiogenesis Induced by Human Hepatocellular CarcinomaGastroenterol Res Pract. 2015

15.) Yuan, C., Wang, P., Zhu, L., Dissanayaka, W.L., Green, D.W., Tong, E.H., Jin, L. & Zhang, C. (2015) Coculture of Stem Cells from Apical Papilla and Human Umbilical Vein Endothelial Cell Under Hypoxia Increases the Formation of Three-Dimensional Vessel-Like Structures in Vitro Tissue Eng Part A. 21

16.) Zhang, E., Guo, Q., Gao, H., Xu, R., Teng, S. & Wu, Y. (2015) Metformin and Resveratrol Inhibited High Glucose-Induced Metabolic Memory of Endothelial Senescence through SIRT1/p300/p53/p21 Pathway PLoS One. 10

17.) Zhao, J., Geng, Y., Hua, H., Cun, B., Chen, Q., Xi, X., Yang, L. & Li, Y. (2015) Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxia Exp Ther Med. 10

18.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells." Med Sci Monit. 17: BR242

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20.) Chen G, Zhao L, Feng J, You G, Sun Q, Li P, Han D, Zhou H. (2013) "Validation of Reliable Reference Genes for Real-Time PCR in Human Umbilical Vein Endothelial Cells on Substrates with Different Stiffness." PLoS One. 8: e67360

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Question:
Greetings! I am the biosafety officer at our institution, and we've had a researcher order primary HUVEC cells from your company. Would you be able to let us know if you test the cultures/isolates for infectious agents or viruses and what agents you test for?

Answers:
Posted by Manoj Sharma on Thursday, August 20, 2015
Each cell strain tests negative for HIV, HBV and HCV. However, keep in mind that diagnostic tests are not necessarily 100% accurate, therefore, proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working with these materials. Never mouth pipette. We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination.
Grizzle WE, Polt S. (1988) “Guidelines to avoid personal contamination by infective agents in research laboratories that use human tissues.” J Tissue Culture Methods. 11: 191-9
Please reply here if you have further questions
Best,
ScienCell Team

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