中枢神经系统的组织由由两类细胞组成，它们可被广泛地分为神经元和神经胶质。神经元是大脑解剖、功能和营养单位。尽管在形状和大小上有很大的可变性，所有的神经元有着共同的形态学特征，这是高度复杂的通信网络中重要的因素。神经元是动力学上的极化细胞并做为神经系统主要的信号单位。大脑含有1×1011个神经元，每一个都与至少10,000个其它神经元相互接触。
推荐培养基: （NM, Cat. No. 1521）
| 货号 | R1540 |
| 产地 | 美国 |
| 缩写 | RN-h |
| 规格 |
1 x 10^6 cells/vial |
| 用途 | 科研 |
| 运输 | 干冰 |
| 保存 | 液氮 |
Typically for cells seeded at 30,000 cells/cm2 in 96-well plate, how many days of culture is required before the cells form neurites and reach ~70% confluency?
Dear Kelly,
The Rat Neurons-hippocampal should start forming neurites after 1 day after thawing the cells. The neurons are non-proliferative cells, so the cells will not proliferate. Cells need to be plated at the density you would like them for your experiments as the cells will not proliferate. Thanks!
Hi, May I ask if I thaw the cells in a tissue culture flask, can I subsequently trypsinize the cells and seed in 96-well plates for my assay? Thanks!
Hi Shan,
Thanks for asking! We recommend you to plate the Rat Neurons-hippocampal directly in 96-well plate that is cell culture treated so the neurons attach to the plate, rather than plating first in a flask and then moving the cells. These cells do not proliferate, so it is best to seed them onto the 96-well immediately after thawing the cells. Thanks!
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