您是第 10179388 位 欢迎访问 中乔新舟官网 ! 全国免费电话:400-038-9959 我的购物车(0) 注 册 / 登 录
扫码关注公众号
您当前的位置:首页 > 产品中心

人IGF-1酶联免疫试剂盒

英文名: Human IGF-1 ELISA Kit
货号:EK0376
价格:¥5265.00
加入购物车,提交订单信息之后,我们会第一时间与您取得联系!
  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

人IGF-1酶联免疫试剂盒描述:


       Insulin-like growth factor 1 (IGF-1), also known as somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF-1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF-1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF-1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF-1A). The structure of IGF-1 resembles that of IGF-2. And the IGF-1 and IGF-2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of six binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.


       ScienCell's human IGF-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IGF-1-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added, and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human IGF-1 captured in strips.


产品使用说明:仅供科研研究使用。


 

货号

EK0376

产地

美国

缩写

hIGF-1-ELISA

规格

96 tests

用途

科研

储存

频繁使用储存在4°C,很少使用储存在-20°C.

运输

胶冰

No Publication available at this time


Question:

       Hi, I was wondering if the buffer in this kit inhibits IGFBPs. Do you use a specific protocol to free IGF-1 when preparing tissue lysates?


Answers:


       Hi Patricia,


       EK0376 is unavailable at the moment. The kit can recognize free IGF-1 and IGFBPs.


       thank you for your interest in Sciencell Products

公司简介 / Company profile
上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
           上海中乔新舟生物科技有限公司(官网:www.zqx...
联系我们 / Contact
电 话:021-56760357;021-56760351
邮 箱:sales@zqxzbio.com
邮 编:200439
地 址:上海市宝山区长江南路180号
Copyright © 2014 ZQXZBIO All rights reserved. 网站地图

沪公网安备 31011002001038号

技术支持:攸攸网络 沪ICP备14008091号