人IL-1β酶联免疫试剂盒描述:
Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14 and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, causes oligodendrocyte death in co-culture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR co-repressor complex, resulting in de-repression of a specific subset of nuclear factor-kappa-B-regulated genes. Furthermore, microenvironmental IL-1β and, to a lesser extent, IL-1a, are required for in vivo angiogenesis and invasiveness of different tumor cells. Additionally, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
ScienCell's human IL-1β ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IL1-β-specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human IL-1β captured in strips. The human IL-1β detected in sera, plasma, body fluids, tissue lysates or cell culture supernatants with this ELISA kit is mainly activated mature protein.
产品使用说明:仅供科研研究使用。
货号 |
EK0392 |
产地 |
美国 |
缩写 |
hIL-1β-ELISA |
规格 |
96 tests |
用途 |
科研 |
保存 |
频繁使用储存在4°C,很少使用储存在-20°C. |
运输 |
胶冰 |
参考文献 |
1. Takahashi, J. L.; Giuliani, F.; Power, C.;
Imai, Y.; Yong, V. W. : Interleukin-1-beta promotes oligodendrocyte death
through glutamate excitotoxicity. Ann. Neurol. 53: 588-595, 2003. |
2. Baek, S. H.; Ohgi, K. A.; Rose, D. W.; Koo, E. H.; Glass, C. K.; Rosenfeld, M. G. : Exchange of N-CoR corepressor and Tip60 coactivator complexes links gene expression by NF-kappa-B and beta-amyloid precursor protein. Cell 110: 55-67, 2002.
3. Voronov, E.; Shouval, D. S.; Krelin, Y.; Cagnano, E.; Benharroch, D.; Iwakura, Y.; Dinarello, C. A.; Apte, R. N. : IL-1 is required for tumor invasiveness and angiogenesis. Proc. Nat. Acad. Sci. 100: 2645-2650, 2003.
4. Chen, C.-N.; Li, Y.-S. J.; Yeh, Y.-T.; Lee, P.-L.; Usami, S.; Chien, S.; Chiu, J.-J. : Synergistic roles of platelet-derived growth factor-BB and interleukin-1-beta in phenotypic modulation of human aortic smooth muscle cells. Proc. Nat. Acad. Sci. 103: 2665-2670, 2006.
货号 | 产品名称 | 规格 | 价格 | 指令 |
EK0349 | 人纤维连接蛋白酶联免疫试剂盒 | 96 tests | ¥4005.00 | 放入购物车 》 |
EK0360 | 人粒细胞集落刺激因子酶联免疫试剂盒 | 96 tests | ¥4365.00 | 放入购物车 》 |
EK0364 | 人粒细胞巨噬细胞刺激因子酶联免疫试剂盒 | 96 tests | ¥7020.00 | 放入购物车 》 |
EK0373 | 人IFN-γ酶联免疫试剂盒 | 96 tests | ¥4365.00 | 放入购物车 》 |
EK0382 | 人IGFBP-1酶联免疫试剂盒 | 96 tests | ¥6120.00 | 放入购物车 》 |
EK0397 | 人 IL-2酶联免疫试剂盒 | 96 tests | ¥4365.00 | 放入购物车 》 |