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人 MMP-1酶联免疫试剂盒

英文名: Human MMP-1 ELISA Kit
货号:EK0458
价格:¥6660.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

人 MMP-1酶联免疫试剂盒描述:


       Matrix metalloproteinase-1 (MMP-1), commonly known as collagenase, is a member of the matrix metalloproteinases (MMPs) family. These are zinc-dependent proteases that degrade extracellular matrix proteins. The MMPs comprise of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. MMP-1 plays an important role in the degradation of collagen in inflammatory diseases. MMP-1 (interstitial collagenase), MMP-9 (gelatinase B) and MMP-12 (macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53 KDa. The detected MMP-1 includes zymogen and active enzyme.


       ScienCell's human MMP-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human MMP-1-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human MMP-1 captured in strips.


产品使用说明:仅供科研研究使用

货号

EK0458

产地

美国

缩写

hMMP-1-ELISA

规格

96 tests

用途

科研

保存

频繁使用储存在4°C,很少使用储存在-20°C.

运输

胶冰

参考文献

1. Domeij, H.; Modeer, T.; Quezada, HC.; Yucel-Lindberg, T. Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1. Biochem. Biophys. Res. Commun. 2005 Dec 30;338(4):1825-33. Epub 2005 Nov 2. 
2. Joos, L.; He, J.-Q.; Shepherdson, M. B.; Connett, J. E.; Anthonisen, N. R.; Pare, P. D.; Sandford, A. J. The role of matrix metalloproteinase polymorphisms in the rate of decline in lung function. Hum. Molec. Genet. 11: 569-576, 2002.
 
3. Lahmann, C.; Bergemann, J.; Harrison, G.; Young, A. R. Matrix metalloproteinase-1 and skin ageing in smokers. (Letter) Lancet 357: 935-936, 2001.

       1. Domeij, H.; Modeer, T.; Quezada, HC.; Yucel-Lindberg, T. Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1. Biochem. Biophys. Res. Commun. 2005 Dec 30;338(4):1825-33. Epub 2005 Nov 2. 


       2. Joos, L.; He, J.-Q.; Shepherdson, M. B.; Connett, J. E.; Anthonisen, N. R.; Pare, P. D.; Sandford, A. J. The role of matrix metalloproteinase polymorphisms in the rate of decline in lung function. Hum. Molec. Genet. 11: 569-576, 2002. 


       3. Lahmann, C.; Bergemann, J.; Harrison, G.; Young, A. R. Matrix metalloproteinase-1 and skin ageing in smokers. (Letter) Lancet 357: 935-936, 2001.

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