名称 |
Sf9昆虫细胞系 |
货号 |
ZQ0497 |
产品介绍 |
Sf9细胞是源于雌性草地贪夜蛾蛹的卵巢组织,可以用于复制杆状病毒表达载体。 |
种属 |
草地贪夜蛾 |
组织来源 |
原位;卵巢 |
形态学 |
圆形、附着、上皮 |
细胞类型 |
自发永生化细胞系 |
倍增时间 |
25.2 +- 2.8 hours (Note=At 28 Celsius) (PubMed=10718368); 27 hours (PubMed=21667340); ~50 hours (DSMZ=ACC-125) |
生长方式 |
贴壁 |
培养基和添加剂 |
|
推荐完全培养基货号 |
ZM0497 |
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
ATCC; CRL-1711 BCRC; 60011 CCTCC; GDC0008 CCLV; CCLV-RIE 0352 CLS; 604328 DSMZ; ACC-125 ECACC; 89070101 IBRC; C10127 IZSLER; BS CL 157 KCB; KCB 92018YJ KCB; KCB 200807YJ NCBI_Iran; C425 NISES; SK0002 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0497 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=1367489; DOI=10.1021/bp00007a002
Hink W.F., Thomsen D.R., Davidson D.J., Meyer A.L., Castellino F.J.
Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines.
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Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.
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Comparative recombinant protein production of eight insect cell lines.
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Gerbal M., Fournier P., Barry P., Mariller M., Odier F., Devauchelle G., Duonor-Cerutti M.
Adaptation of an insect cell line of Spodoptera frugiperda to grow at 37 degrees C: characterization of an endodiploid clone.
In Vitro Cell. Dev. Biol. Anim. 36:117-124(2000)
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Grasela J.J., McIntosh A.H., Goodman C.L., Wilson L.E., King L.A.
Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 36:205-210(2000)
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Iwahori S., Ikeda M., Kobayashi M.
Comparative characterization of pcna genes from Hyphantria cunea nucleopolyhedrovirus, and two cell lines from the fall armyworm, Spodoptera frugiperda, and the fall webworm, Hyphantria cunea.
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PubMed=12052082; DOI=10.1021/bp020028
Jarman-Smith R.F., Armstrong S.J., Mannix C.J., Al-Rubeai M.
Chromosome instability in Spodoptera frugiperda Sf-9 cell line.
Biotechnol. Prog. 18:623-628(2002)
PubMed=14668217; DOI=10.1093/bioinformatics/btg324
Landais I., Ogliastro M., Mita K., Nohata J., Lopez-Ferber M., Duonor-Cerutti M., Shimada T., Fournier P., Devauchelle G.
Annotation pattern of ESTs from Spodoptera frugiperda Sf9 cells and analysis of the ribosomal protein genes reveal insect-specific features and unexpectedly low codon usage bias.
Bioinformatics 19:2343-2350(2003)
PubMed=19003226; DOI=10.1023/B:CYTO.0000043394.40309.48
Jarman-Smith R.F., Mannix C.J., Al-Rubeai M.
Characterisation of tetraploid and diploid clones of Spodoptera frugiperda cell line.
Cytotechnology 44:15-25(2004)
PubMed=15926859; DOI=10.1290/0412081.1
Chen Y.P., Gundersen-Rindal D.E., Lynn D.E.
Baculovirus-based expression of an insect viral protein in 12 different insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 41:43-49(2005)
DOI=10.3923/jas.2007.4040.4043
Alhag S.K.N., Yao H.-C., Peng J.-X.
Identification of insect cell lines from 8 lepidopteran species by DNA amplification fingerprinting.
J. Appl. Sci. 7:4040-4044(2007)
DOI=10.1111/j.1439-0418.2010.01574.x
Wu C.-Y., Lin H.-F., Wang C.-H., Lo C.-F.
Identification of insect cell lines and cell-line cross-contaminations by nuclear ribosomal ITS sequences.
J. Appl. Entomol. 135:601-610(2010)
PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)
PubMed=20300881; DOI=10.1007/s12033-010-9268-3
Krammer F., Schinko T., Palmberger D., Tauer C., Messner P., Grabherr R.
Trichoplusia ni cells (High Five) are highly efficient for the production of influenza A virus-like particles: a comparison of two insect cell lines as production platforms for influenza vaccines.
Mol. Biotechnol. 45:226-234(2010)
PubMed=21667340; DOI=10.1007/s12250-011-3177-x
Wu Y.-L., Jiang L., Hashimoto Y., Granados R.R., Li G.-X.
Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines.
Virol. Sin. 26:198-205(2011)
PubMed=23891577; DOI=10.1016/j.tiv.2013.07.007
Curtis T.M., Collins A.M., Gerlach B.D., Brennan L.M., Widder M.W., van der Schalie W.H., Vo N.T.K., Bols N.C.
Suitability of invertebrate and vertebrate cells in a portable impedance-based toxicity sensor: temperature mediated impacts on long-term survival.
Toxicol. In Vitro 27:2061-2066(2013)
PubMed=24375231; DOI=10.1007/s10529-013-1429-6
Wilde M., Klausberger M., Palmberger D., Ernst W., Grabherr R.
Tnao38, high five and Sf9 -- evaluation of host-virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression.
Biotechnol. Lett. 36:743-749(2014)
PubMed=24672045; DOI=10.1128/JVI.00780-14
Ma H.-L., Galvin T.A., Glasner D.R., Shaheduzzaman S., Khan A.S.
Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines.
J. Virol. 88:6576-6585(2014)
PubMed=28423032; DOI=10.1371/journal.pone.0175633
Hashimoto Y., Macri D., Srivastava I., McPherson C., Felberbaum R., Post P., Cox M.M.J.
Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line.
PLoS ONE 12:E0175633-E0175633(2017)
PubMed=28839023; DOI=10.1128/genomeA.00829-17
Nandakumar S., Ma H.-L., Khan A.S.
Whole-genome sequence of the Spodoptera frugiperda Sf9 insect cell line.
Genome Announc. 5:e00829.17.1-e00829.17.2(2017)
PubMed=29038528; DOI=10.1038/s41598-017-12713-9
Shu B.-S., Zhang J.-J., Sethuraman V., Cui G.-F., Yi X., Zhong G.-H.
Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by azadirachtin.
Sci. Rep. 7:13231-13231(2017)
PubMed=29133148; DOI=10.1016/j.pep.2017.11.002
Geisler C., Jarvis D.L.
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2017)