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Sf21昆虫卵巢细胞系(暂不提供)

英文名:Sf21
货号:ZQ0499
价格:¥1700.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

名称

Sf21昆虫卵巢细胞系

货号

ZQ0499

产品介绍

 

种属

草地贪夜蛾

组织来源

原位;卵巢

形态学


90% 圆形细胞;10% 纺锤形,大部分贴壁,少数细胞处于悬浮状态

细胞类型

自发永生化细胞系

倍增时间

 26-30 hours (PubMed=68913); 33 +- 1 hours (Note=In static culture), 26 +- 1 hours (Note=In suspension culture) (PubMed=10585188); ~3 days (DSMZ=ACC-119); 24 hours (ECACC=05022801)

生长方式

半悬浮

培养基和添加剂

TNM-SH(or Grace's) +10%FBS(中乔新舟  货号:ZQ500-A+1%双抗(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0499

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;37℃

保藏机构

CCTCC; GDC0020

DSMZ; ACC-119

ECACC; 05022801

KCB; KCB 2010008YJ

NCBI_Iran; C503

供应限制

仅供科研使用


货号

ZQ0499

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


DOI=10.1016/B978-0-12-709902-6.50017-0
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A catalog of invertebrate cell lines.
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PubMed=68913; DOI=10.1007/BF02615077
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The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae).
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Volkman L.E., Goldsmith P.A.
Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro.
Appl. Environ. Microbiol. 44:227-233(1982)

CLPUB00492
Mitsuhashi J.
Nutritional requirements of insect cells in vitro.
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PubMed=1367489; DOI=10.1021/bp00007a002
Hink W.F., Thomsen D.R., Davidson D.J., Meyer A.L., Castellino F.J.
Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines.
Biotechnol. Prog. 7:9-14(1991)

PubMed=1369220; DOI=10.1021/bp00017a003
Wickham T.J., Davis T., Granados R.R., Shuler M.L., Wood H.A.
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PubMed=8314732; DOI=10.1007/BF02633986
Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L., Wood H.A.
Comparative recombinant protein production of eight insect cell lines.
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CLPUB00177
Li L.-L., Chen Q.-H.
Storage of insect cell lines by a simple method.
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PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x
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PubMed=10475266; DOI=10.1007/s11626-999-0010-z
Gundersen-Rindal D.E., Lynn D.E., Dougherty E.M.
Transformation of lepidopteran and coleopteran insect cell lines by Glyptapanteles indiensis polydnavirus DNA.
In Vitro Cell. Dev. Biol. Anim. 35:111-114(1999)

PubMed=10585188; DOI=10.1021/bp990119f
Keith M.B., Farrell P.J., Iatrou K., Behie L.A.
Screening of transformed insect cell lines for recombinant protein production.
Biotechnol. Prog. 15:1046-1052(1999)

PubMed=10777062; DOI=10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2
Grasela J.J., McIntosh A.H., Goodman C.L., Wilson L.E., King L.A.
Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 36:205-210(2000)

PubMed=10852353; DOI=10.1290/1071-2690(2000)036<0271:IVHRSW>2.0.CO;2
Kariuki C.W., McIntosh A.H., Goodman C.L.
In vitro host range studies with a new baculovirus isolate from the diamondback moth Plutella xylostella (L.) (Plutellidae: Lepidoptera).
In Vitro Cell. Dev. Biol. Anim. 36:271-276(2000)

PubMed=12623313; DOI=10.1016/S0022-2011(03)00002-8
Lynn D.E.
Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses.
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PubMed=15038778; DOI=10.1290/1543-706X(2003)039<0353:AOISRT>2.0.CO;2
Grasela J.J., McIntosh A.H.
Application of inter-simple sequence repeats to insect cell lines: identification at the clonal and tissue-specific level.
In Vitro Cell. Dev. Biol. Anim. 39:353-363(2003)

PubMed=15926859; DOI=10.1290/0412081.1
Chen Y.P., Gundersen-Rindal D.E., Lynn D.E.
Baculovirus-based expression of an insect viral protein in 12 different insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 41:43-49(2005)

PubMed=16409117; DOI=10.1290/0412083R.1
McIntosh A.H., Grasela J.J., Popham H.J.R.
AcMNPV in permissive, semipermissive, and nonpermissive cell lines from Arthropoda.
In Vitro Cell. Dev. Biol. Anim. 41:298-304(2005)

PubMed=22797937; DOI=10.1007/s10158-012-0138-5
Jenson L.J., Paulson S.L., Bloomquist J.R.
Induction and inhibition of an apparent neuronal phenotype in Spodoptera frugiperda insect cells (Sf21) by chemical agents.
Invertebr. Neurosci. 12:119-127(2012)

PubMed=24672045; DOI=10.1128/JVI.00780-14
Ma H.-L., Galvin T.A., Glasner D.R., Shaheduzzaman S., Khan A.S.
Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines.
J. Virol. 88:6576-6585(2014)

PubMed=29133148; DOI=10.1016/j.pep.2017.11.002
Geisler C., Jarvis D.L.
Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2017)

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