名称 |
Sf21昆虫卵巢细胞系 |
货号 |
ZQ0499 |
产品介绍 |
|
种属 |
草地贪夜蛾 |
组织来源 |
原位;卵巢 |
形态学 |
|
细胞类型 |
自发永生化细胞系 |
倍增时间 |
26-30 hours (PubMed=68913); 33 +- 1 hours (Note=In static culture), 26 +- 1 hours (Note=In suspension culture) (PubMed=10585188); ~3 days (DSMZ=ACC-119); 24 hours (ECACC=05022801) |
生长方式 |
半悬浮 |
培养基和添加剂 |
TNM-SH(or Grace's) +10%FBS(中乔新舟 货号:ZQ500-A)+1%双抗(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
ZM0499 |
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
CCTCC; GDC0020 DSMZ; ACC-119 ECACC; 05022801 KCB; KCB 2010008YJ NCBI_Iran; C503 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0499 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
DOI=10.1016/B978-0-12-709902-6.50017-0
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CLPUB00492
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PubMed=10585188; DOI=10.1021/bp990119f
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Screening of transformed insect cell lines for recombinant protein production.
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PubMed=10777062; DOI=10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2
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Expression of the green fluorescent protein carried by Autographa californica multiple nucleopolyhedrovirus in insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 36:205-210(2000)
PubMed=10852353; DOI=10.1290/1071-2690(2000)036<0271:IVHRSW>2.0.CO;2
Kariuki C.W., McIntosh A.H., Goodman C.L.
In vitro host range studies with a new baculovirus isolate from the diamondback moth Plutella xylostella (L.) (Plutellidae: Lepidoptera).
In Vitro Cell. Dev. Biol. Anim. 36:271-276(2000)
PubMed=12623313; DOI=10.1016/S0022-2011(03)00002-8
Lynn D.E.
Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses.
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Grasela J.J., McIntosh A.H.
Application of inter-simple sequence repeats to insect cell lines: identification at the clonal and tissue-specific level.
In Vitro Cell. Dev. Biol. Anim. 39:353-363(2003)
PubMed=15926859; DOI=10.1290/0412081.1
Chen Y.P., Gundersen-Rindal D.E., Lynn D.E.
Baculovirus-based expression of an insect viral protein in 12 different insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 41:43-49(2005)
PubMed=16409117; DOI=10.1290/0412083R.1
McIntosh A.H., Grasela J.J., Popham H.J.R.
AcMNPV in permissive, semipermissive, and nonpermissive cell lines from Arthropoda.
In Vitro Cell. Dev. Biol. Anim. 41:298-304(2005)
PubMed=22797937; DOI=10.1007/s10158-012-0138-5
Jenson L.J., Paulson S.L., Bloomquist J.R.
Induction and inhibition of an apparent neuronal phenotype in Spodoptera frugiperda insect cells (Sf21) by chemical agents.
Invertebr. Neurosci. 12:119-127(2012)
PubMed=24672045; DOI=10.1128/JVI.00780-14
Ma H.-L., Galvin T.A., Glasner D.R., Shaheduzzaman S., Khan A.S.
Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines.
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PubMed=29133148; DOI=10.1016/j.pep.2017.11.002
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Adventitious viruses in insect cell lines used for recombinant protein expression.
Protein Expr. Purif. 144:25-32(2017)