产品名称	WI-38 VA13 subline 2RA，WI-38 VA13亚系2RA  SV-40转化肺成纤维细胞
货号	ZQ0538
产品介绍	WI-38 VA13 subline 2RA（简称2RA），该细胞系衍生自WI-38细胞。这个细胞系是在1964年由Girardi等人从一位3个月胎龄的女性胚胎的肺成纤维细胞中建立经过SV-40病毒转化的成纤维细胞系。WI-38细胞感染低滴度的SV40病毒，经过连续传代、克隆选择获得多株亚系，其中2RA亚系含有SV40neo(T)和转移抗原，从而改变其有限细胞系的特性，可在常规条件下连续传代。 2RA细胞系广泛用于生物医学研究，包括病毒学、细胞生物学和分子生物学研究。由于其转化特性，2RA细胞系也可用于疫苗开发和基因功能研究。该细胞系也适用于研究细胞信号传导、细胞凋亡、细胞周期控制和癌症机理。
种属	人
性别/年龄	女性/3个月
组织	肺
疾病	常态
细胞类型	转化细胞系
形态学	成纤维细胞
生长方式	贴壁
倍增时间	大约24~48小时
培养基和添加剂	MEM（含NEAA）培养基(中乔新舟  货号:ZQ-300)+10%FBS（品牌：中乔新舟  货号：ZQ500-A）+1%P/S（中乔新舟  货号：CSP006）
推荐完全培养基货号	ZM0538
生物安全等级	BSL-2
STR位点信息	Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 11,12 D5S818: 10 D7S820: 9,11 TH01: 9.3 TPOX: 8 vWA: 19,20 D3S1358: 16,17 D21S11: 30,30.2 D18S51: 16,18 Penta_E: 13,14 Penta_D: 13 D8S1179: 14 FGA: 22,24 D19S433: 13,16.2 D2S1338: 19,25
培养条件	95%空气，5%二氧化碳；37℃
抗原表达/受体表达	***
基因表达	***
保藏机构	ATCC
供应限制	仅供科研使用

货号	ZQ0538
发货规格	活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一
发货形式	活细胞：常温运输；冻存管：干冰运输
储存温度	活细胞：培养箱；冻存管：液氮罐
产地	中国
供应限制	仅供科研使用

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