名称 |
Marc145长尾绿猴肾上皮细胞(种属鉴定) |
货号 |
ZQ0797 |
产品介绍 |
该细胞系来源于一非洲绿猴的胚胎肾组织。 |
种属 |
长尾绿猴 |
组织来源 |
原位;胎肾 |
形态学 |
上皮样 |
细胞类型 |
自发永生化细胞系 |
倍增时间 |
|
生长方式 |
贴壁 |
培养基和添加剂 |
DMEM(中乔新舟 货号:ZQ-100)+15%FBS(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
CCLV; CCLV-RIE 0277 CLS; 305006 IZSLER; BS CL 127 KCB; KCB 200801YJ |
供应限制 |
仅供科研使用 |
货号 |
ZQ0797 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
原文链接: https://www.nature.com/articles/s41467-022-28920-6
PubMed=8257302; DOI=10.1007/BF01313785
Kim H.S., Kwang J., Yoon I.J., Joo H.S., Frey M.L.
Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line.
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Patent=US5690940
Joo H.S.
Low pathogencity PRRS live virus vaccines and methods of preparation thereof.
Patent number US5690940, 25-Nov-1997
PubMed=17081295; DOI=10.1186/1743-422X-3-90
Cafruny W.A., Duman R.G., Wong G.H.W., Said S., Ward-Demo P., Rowland R.R.R., Nelson E.A.
Porcine reproductive and respiratory syndrome virus (PRRSV) infection spreads by cell-to-cell transfer in cultured MARC-145 cells, is dependent on an intact cytoskeleton, and is suppressed by drug-targeting of cell permissiveness to virus infection.
Virol. J. 3:90.1-90.17(2006)
PubMed=23148668; DOI=10.1186/1743-422X-9-267
Provost C., Jia J.-J., Music N., Levesque C., Lebel M.-E., del Castillo J.R.E., Jacques M., Gagnon C.A.
Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line.
Virol. J. 9:267.1-267.14(2012)
PubMed=28046048; DOI=10.1371/journal.pone.0169391
Dotti S., Lombardo T., Villa R., Cacciamali A., Zanotti C., Andreani N.A., Cinotti S., Ferrari M.
Transformation and tumorigenicity testing of simian cell lines and evaluation of poliovirus replication.
PLoS ONE 12:E0169391-E0169391(2017)
PubMed=29682543; DOI=10.1155/2018/4315861
Wu X.-J., Qi J., Cong X.-Y., Chen L., Hu Y., Yoo D.-W., Wang G.-S., Tian F.-L., Li F., Sun W.-B., Chen Z., Guo L.-H., Wu J.-Q., Li J., Wang J.-B., Zhao X.-M., Du Y.-J.
Establishment and characterization of a high and stable porcine CD163-expressing MARC-145 cell line.
BioMed Res. Int. 2018:4315861.1-4315861.9(2018)
PubMed=33268540; DOI=10.1128/jcm.01757-20
Yim-im W., Huang H.-Y., Park J., Wang C., Calzada-Nova G., Gauger P., Harmon K., Main R., Zhang J.-Q.
Comparison of ZMAC and MARC-145 cell lines for improving porcine reproductive and respiratory syndrome virus isolation from clinical samples.
J. Clin. Microbiol. 59:e01757.20.1-e01757.20.15(2021)
PubMed=34205087; DOI=10.3390/vaccines9060594
Xie J.-X., Vereecke N., Theuns S., Oh D.-Y., Vanderheijden N., Trus I., Sauer J., Vyt P., Bonckaert C., Lalonde C., Provost C., Gagnon C.A., Nauwynck H.J.
Comparison of primary virus isolation in pulmonary alveolar macrophages and four different continuous cell lines for type 1 and type 2 porcine reproductive and respiratory syndrome virus.
Vaccines (Basel) 9:594.1-594.13(2021)
PubMed=34737324; DOI=10.1038/s41598-021-00779-5
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)