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RPMI-8226人多发性骨髓瘤细胞(STR鉴定)

英文名:RPMI-8226
货号:ZQ0903
价格:¥1800.00
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推荐组合

RPMI-8226人多发性骨髓瘤细胞(STR鉴定)

¥1800.00
+

RPMI 8226人多发性骨肉瘤细胞专用培养基

¥350.00 ¥450.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥2150 ¥2450.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

RPMI-8226人多发性骨髓瘤细胞

货号

ZQ0903

产品介绍

RPMI 8226 细胞是一种人骨髓瘤细胞系,于 1966 年从一名 61 岁男性多发性骨髓瘤患者的外周血中建立。该细胞系以其开发地罗斯威尔帕克纪念研究所(RPMI)命名,数字8226表示其在细胞库中的具体目录号。该细胞系是研究多发性骨髓瘤以及浆细胞生物学、免疫学研究和癌症治疗相关方面的重要模型系统。这种细胞系的特点是能够产生和分泌lambda类型的免疫球蛋白的 kappa 轻链,但不分泌重链。这一特性经常在研究中用于研究抗体的产生和分泌机制。
RPMI 8226 细胞表现出许多染色体异常,这是多发性骨髓瘤细胞的典型特征。这些包括影响各种癌基因和抑癌基因的易位、缺失和扩增。
RPMI-8226细胞通常用于多发性骨髓瘤的病理机制研究、药物筛选与开发、免疫学研究,并且是合适的转染宿主细胞。
总之,RPMI 8226 细胞为多发性骨髓瘤研究提供了关键的体外模型,使研究这种疾病的生物学和分子机制以及开发治疗策略成为可能。

注意事项:
RPMI 8226细胞悬浮聚小团生长,细胞复苏生长至传代需要一周左右。

种属

性别/年龄

男/61岁

组织

外周血

疾病

浆细胞瘤

细胞类型

B淋巴细胞

形态学

成淋巴细胞

生长方式

单个悬浮,有部分贴壁细胞(有时可达到50%)

倍增时间

大约48~168小时 

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200+10%胎牛血清(中乔新舟  货号:ZQ0500)+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0903

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y

CSF1PO: 12

D13S317: 11

D16S539: 9

D5S818: 11,13

D7S820: 9,10

THO1: 8

TPOX: 8,11

vWA: 16,18

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

  ***

基因表达

  ***

保藏机构

ATCC; CCL-155 ATCC; CRM-CCL-155 BCRC; 60384 DSMZ; ACC-402 

ECACC; 87012702 

供应限制

仅供科研使用

货号

ZQ0903

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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PubMed=23933261; DOI=10.1016/j.celrep.2013.07.018
Moghaddas Gholami A., Hahne H., Wu Z.-X., Auer F.J., Meng C., Wilhelm M., Kuster B.
Global proteome analysis of the NCI-60 cell line panel.
Cell Rep. 4:609-620(2013)

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