您是第 11222889 位 欢迎访问 中乔新舟官网 ! 全国免费电话:400-038-9959 我的购物车(0) 注 册 / 登 录
扫码关注公众号
您当前的位置:首页 > 产品中心

A2780人卵巢癌细胞

英文名:A2780
货号:ZQ0486
价格:¥1350.00
加入购物车,提交订单信息之后,我们会第一时间与您取得联系!
推荐组合

A2780人卵巢癌细胞

¥1350.00
+

A2780人卵巢癌细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥1700 ¥2680.00
加入购物车
  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

A2780人卵巢癌细胞

货号

ZQ0486

产品介绍

A2780细胞系最初是从ECACC获得的,其细胞形态为上皮细胞样,生长特性为贴壁生长。该细胞被分离自一名未经治疗的卵巢癌患者的肿瘤组织,在建立过程中未受到抗癌药物或化学物质的影响。在支原体检测中呈阴性。
A2780细胞系以单层和悬浮液的形式在旋转培养器中生长,被广泛应用于毒性试验和癌症基因研究。此外该细胞系已被用于多项研究,包括探索蜗牛和蛞蝓在卵巢肿瘤新生血管形成中的作用,以及应用脂质体克服卵巢癌细胞对顺铂的耐药性的研究。研究也证实了NMDA(N-甲基-D-天冬氨酸)受体作为治疗卵巢癌的潜在靶点的意义,以及低分子肝素对卵巢癌细胞顺铂耐药性的影响。
A2780细胞在癌症治疗和耐药机制研究中被广泛应用。由于其来自未经治疗的患者,A2780细胞系成为了评估癌症治疗效果、研究治疗耐药性以及探索新药物疗法的重要模型之一。

A2780细胞的亲本株A2780还衍生出顺铂耐药细胞系A2780 cis和阿霉素耐药细胞系A2780 ADR,为进一步研究耐药机制提供了有力工具。

种属

性别/年龄

组织

卵巢

疾病

卵巢子宫内膜样腺癌 

细胞类型

肿瘤细胞 

形态学

上皮的

生长方式

贴壁和悬浮,混合生长

倍增时间

大约16~26.3小时

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200)+2 mM L-谷氨酰胺(中乔新舟 货号:CSP012)+1 mM 丙酮酸钠(中乔新舟 货号:CSP003)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%双抗(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0486

生物安全等级

BSL-1

STR位点信息

Amelogenin:X
CSF1PO: 10,11
D2S1338:21,22
D3S1358:14,16
D5S818: 11,12
D7S820: 10
D8S1179:15,17
D13S317:12,13
D16S539:11,12,13 (ICLC=HTL98008)
                11,13 (AddexBio=C0017002/20; Cosmic-CLP=906804;                                                 ECACC=93112519; PubMed=25230021; PubMed=25877200;                               PubMed=30485824)
D18S51: 16,18
D19S433:12
D21S11: 28 (PubMed=25230021; PubMed=25877200)
               28,29 (PubMed=30485824)
FGA:19,24
Penta D: 8,9
Penta E: 10,13
TH01:6
TPOX:8,10
vWA:15,16。 

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ECACC; 93112519

供应限制

仅供科研使用


货号

ZQ0486

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


PubMed=6954533; DOI=10.1073/pnas.79.7.2194
Westin E.H., Gallo R.C., Arya S.K., Eva A., Souza L.M., Baluda M.A., Aaronson S.A., Wong-Staal F.
Differential expression of the amv gene in human hematopoietic cells.
Proc. Natl. Acad. Sci. U.S.A. 79:2194-2198(1982)


PubMed=6372095; DOI=10.1126/science.6372095
Rogan A.M., Hamilton T.C., Young R.C., Klecker R.W. Jr., Ozols R.F.
Reversal of adriamycin resistance by verapamil in human ovarian cancer.
Science 224:994-996(1984)


PubMed=3986765
Louie K.G., Behrens B.C., Kinsella T.J., Hamilton T.C., Grotzinger K.R., McKoy W.M., Winker M.A., Ozols R.F.
Radiation survival parameters of antineoplastic drug-sensitive and -resistant human ovarian cancer cell lines and their modification by buthionine sulfoximine.
Cancer Res. 45:2110-2115(1985)


PubMed=2429947; DOI=10.20772/cancersci1985.77.9_941
Tsuruo T., Hamilton T.C., Louie K.G., Behrens B.C., Young R.C., Ozols R.F.
Collateral susceptibility of adriamycin-, melphalan- and cisplatin-resistant human ovarian tumor cells to bleomycin.
Jpn. J. Cancer Res. 77:941-945(1986)


PubMed=3539322
Behrens B.C., Hamilton T.C., Masuda H., Grotzinger K.R., Whang-Peng J., Louie K.G., Knutsen T., McKoy W.M., Young R.C., Ozols R.F.
Characterization of a cis-diamminedichloroplatinum(II)-resistant human ovarian cancer cell line and its use in evaluation of platinum analogues.
Cancer Res. 47:414-418(1987)


PubMed=3335022
Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)


PubMed=2751261
Mazzoni A., Trave F., Canti G., Franco P.
Effect of the tiapamil analog RO11-2933 on cellular sensitivity to antitumor drugs in sensitive and multidrug resistant human ovarian cancer cells.
Anticancer Res. 9:367-371(1989)


PubMed=1999494; DOI=10.1172/JCI115080
Parker R.J., Eastman A., Bostick-Bruton F., Reed E.
Acquired cisplatin resistance in human ovarian cancer cells is associated with enhanced repair of cisplatin-DNA lesions and reduced drug accumulation.
J. Clin. Invest. 87:772-777(1991)


PubMed=1348364; DOI=10.1073/pnas.89.7.3070
Godwin A.K., Meister A., O'Dwyer P.J., Huang C.-S., Hamilton T.C., Anderson M.E.
High resistance to cisplatin in human ovarian cancer cell lines is associated with marked increase of glutathione synthesis.
Proc. Natl. Acad. Sci. U.S.A. 89:3070-3074(1992)


PubMed=8012063
Cimoli G., Valenti M., Parodi S., Mazzoni A., De Sessa F., Conte P., Russo P.
Reversal of 'atypical'-multidrug resistance by recombinant human tumor necrosis factor in the human ovarian cancer cell line A2780-DX3.
Oncol. Res. 5:311-323(1993)


PubMed=7927910; DOI=10.1002/ijc.2910590118
Huxham I.M., Barlow A.L., Lewis A.D., Plumb J., Mairs R.J., Gaze M.N., Workman P.
Differential intracellular processing of the anthracycline drug ME2303 in doxorubicin-sensitive (A2780) and -resistant (A2780AD) human ovarian cancer cells as studied with confocal laser scanning microscopy and image analysis.
Int. J. Cancer 59:94-102(1994)


PubMed=8557231; DOI=10.1006/gyno.1996.0014
Skilling J.S., Squatrito R.C., Connor J.P., Niemann T., Buller R.E.
p53 gene mutation analysis and antisense-mediated growth inhibition of human ovarian carcinoma cell lines.
Gynecol. Oncol. 60:72-80(1996)


PubMed=9041185
Johnson S.W., Laub P.B., Beesley J.S., Ozols R.F., Hamilton T.C.
Increased platinum-DNA damage tolerance is associated with cisplatin resistance and cross-resistance to various chemotherapeutic agents in unrelated human ovarian cancer cell lines.
Cancer Res. 57:850-856(1997)


PubMed=11103784
Hough C.D., Sherman-Baust C.A., Pizer E.S., Montz F.J., Im D.D., Rosenshein N.B., Cho K.R., Riggins G.J., Morin P.J.
Large-scale serial analysis of gene expression reveals genes differentially expressed in ovarian cancer.
Cancer Res. 60:6281-6287(2000)


PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)


PubMed=12080474; DOI=10.1038/sj.onc.1205542
Manzano R.G., Montuenga L.M., Dayton M., Dent P., Kinoshita I., Vicent S., Gardner G.J., Nguyen P., Choi Y.-H., Trepel J.B., Auersperg N., Birrer M.J.
CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential.
Oncogene 21:4435-4447(2002)


PubMed=15677628; DOI=10.1093/carcin/bgi032
Gorringe K.L., Chin S.-F., Pharoah P.D.P., Staines J.M., Oliveira C., Edwards P.A.W., Caldas C.
Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.
Carcinogenesis 26:923-930(2005)


PubMed=18273836; DOI=10.1002/gcc.20547
Prasad M., Bernardini M., Tsalenko A., Marrano P., Paderova J., Lee C.-H., Ben-Dor A., Barrett M.T., Squire J.A.
High definition cytogenetics and oligonucleotide aCGH analyses of cisplatin-resistant ovarian cancer cells.
Genes Chromosomes Cancer 47:427-436(2008)


PubMed=19714814; DOI=10.1021/pr800856b
Cicchillitti L., Di Michele M., Urbani A., Ferlini C., Donati M.B., Scambia G., Rotilio D.
Comparative proteomic analysis of paclitaxel sensitive A2780 epithelial ovarian cancer cell line and its resistant counterpart A2780TC1 by 2D-DIGE: the role of ERp57.
J. Proteome Res. 8:1902-1912(2009)


PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)


PubMed=20204287; DOI=10.3892/or_00000728
Langland G.T., Yannone S.M., Langland R.A., Nakao A., Guan Y.-H., Long S.B.T., Vonguyen L., Chen D.J., Gray J.W., Chen F.-Q.
Radiosensitivity profiles from a panel of ovarian cancer cell lines exhibiting genetic alterations in p53 and disparate DNA-dependent protein kinase activities.
Oncol. Rep. 23:1021-1026(2010)


PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)


PubMed=22328975; DOI=10.1158/2159-8290.CD-11-0170
Hanrahan A.J., Schultz N., Westfal M.L., Sakr R.A., Giri D.D., Scarperi S., Janakiraman M., Olvera N., Stevens E.V., She Q.-B., Aghajanian C., King T.A., de Stanchina E., Spriggs D.R., Heguy A., Taylor B.S., Sander C., Rosen N., Levine D.A., Solit D.B.
Genomic complexity and AKT dependence in serous ovarian cancer.
Cancer Discov. 2:56-67(2012)


PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)


PubMed=22585861; DOI=10.1158/2159-8290.CD-11-0224
Marcotte R., Brown K.R., Suarez Saiz F.J., Sayad A., Karamboulas K., Krzyzanowski P.M., Sircoulomb F., Medrano M., Fedyshyn Y., Koh J.L.-Y., van Dyk D., Fedyshyn B., Luhova M., Brito G.C., Vizeacoumar F.J., Vizeacoumar F.S., Datti A., Kasimer D., Buzina A., Mero P., Misquitta C., Normand J., Haider M., Ketela T., Wrana J.L., Rottapel R., Neel B.G., Moffat J.
Essential gene profiles in breast, pancreatic, and ovarian cancer cells.
Cancer Discov. 2:172-189(2012)


PubMed=22705003; DOI=10.1016/j.humpath.2012.03.011
Rahman M., Nakayama K., Rahman M.T., Nakayama N., Ishikawa M., Katagiri A., Iida K., Nakayama S., Otsuki Y., Shih I.-M., Miyazaki K.
Clinicopathologic and biological analysis of PIK3CA mutation in ovarian clear cell carcinoma.
Hum. Pathol. 43:2197-2206(2012)


PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017
Korch C.T., Spillman M.A., Jackson T.A., Jacobsen B.M., Murphy S.K., Lessey B.A., Jordan V.C., Bradford A.P.
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)


PubMed=23415752; DOI=10.1016/j.molonc.2012.12.007
Stordal B., Timms K., Farrelly A., Gallagher D., Busschots S., Renaud M., Thery J., Williams D., Potter J., Tran T., Korpanty G., Cremona M., Carey M.S., Li J., Li Y., Aslan O., O'Leary J.J., Mills G.B., Hennessy B.T.
BRCA1/2 mutation analysis in 41 ovarian cell lines reveals only one functionally deleterious BRCA1 mutation.
Mol. Oncol. 7:567-579(2013)


PubMed=23839242; DOI=10.1038/ncomms3126
Domcke S., Sinha R., Levine D.A., Sander C., Schultz N.
Evaluating cell lines as tumour models by comparison of genomic profiles.
Nat. Commun. 4:2126.1-2126.10(2013)

暂无相关产品!
公司简介 / Company profile
上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
      上海中乔新舟生物科技有限公司(官网:www.zqxzbio.com)成立于2011年,历经十多年发展,...
联系我们 / Contact
电 话:021-56760357;021-56760351
邮 箱:sales@zqxzbio.com
邮 编:200439
地 址:上海市宝山区长江南路180号
Copyright © 2014 ZQXZBIO All rights reserved. 网站地图

沪公网安备 31011002001038号

技术支持:攸攸网络 沪ICP备14008091号