产品名称	BT-20人乳腺癌细胞
货号	ZQ0939
产品介绍	BT-20细胞系是1958年从一名74岁白人女性患者的恶性组织中建立的人类乳腺腺癌细胞系。该细胞系表现出上皮样形态，经常用于乳腺癌生物学研究，特别是探索肿瘤生长、基因表达的激素调节以及治疗乳腺癌药物的疗效。 BT-20细胞的特点是在免疫功能低下的小鼠体内植入后能够形成肿瘤，因此可以作为一种有用的乳腺癌体内模型。这些细胞表达雌激素、黄体酮和雄激素的受体，使它们与激素反应途径的研究相关。此外，对BT-20细胞的遗传分析揭示了乳腺癌中常见的基因突变，如TP53和PIK3CA，支持它们在遗传和药理学研究中的应用。 在体外，利用BT-20细胞研究癌细胞增殖、迁移和侵袭的机制。它们也被用来评估化疗药物的细胞毒性，这使得它们对抗癌药物的临床前测试至关重要。BT-20细胞对各种培养条件的适应性及其在体外的强劲生长使其成为癌症研究实验室关注乳腺癌潜在机制和开发新的治疗策略的宝贵资源。
种属	人
性别/年龄	女/74岁
组织	乳房；乳腺
疾病	癌
细胞类型	肿瘤细胞
形态学	上皮样
生长方式	贴壁
倍增时间	70 hours (PubMed=25984343); 66.15 hours (JWGray panel)
培养基和添加剂	DMEM基础培养基（中乔新舟   货号：ZQ-100）+10%FBS（中乔新舟   货号：ZQ0500）+10ug/ml insulin（中乔新舟   货号：CSP001-10）+1%PS（中乔新舟   货号：CSP006）
推荐完全培养基货号	ZM0940
生物安全等级	BSL-1
STR位点信息	Amelogenin: X D3S1358: 17 TH01: 7,9.3 D21S11: 28,29 D18S51: 17 Penta_E: 11,13 D5S818: 12 D13S317: 11 D7S820: 10 D16S539: 11,14 CSF1PO: 12 Penta_D: 10,11 vWA: 16,17 D8S1179: 12 TPOX: 11 FGA: 22,24 D19S433: 15 D2S1338: 19
培养条件	95%空气，5%二氧化碳；37℃
抗原表达/受体表达	文献报道：HLA A1、Bw16（+/-）
基因表达	文献报道：表达WNT3和WNT7B癌基因
保藏机构	ATCC; HTB-19 KCLB; 60061
供应限制	仅供科研使用

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