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人端粒绝对长度和线粒体DNA拷贝数双重定量qPCR检测试剂盒
英文名:Absolute Human Telomere Length and Mitochondrial DNA Copy Number Dual Quantification qPCR Assay Kit
货号:8958
价格:¥9090.00
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人端粒绝对长度和线粒体DNA拷贝数双重定量qPCR检测试剂盒描述:   


      端粒是染色体末端重复的核苷酸元素,保护染色体免于退化和遗传信息丢失。正常的二倍体细胞在每个细胞周期中都会失去端粒。因此,端粒长度会随着时间的推移而减少,并可能预测寿命。准确和一致的端粒长度定量在细胞生物学的许多方面,如染色体不稳定、DNA修复、衰老、凋亡、细胞功能障碍和肿瘤发生中都很重要。线粒体DNA (mtDNA)是一种环状、多染色体的基因组DNA,位于线粒体中,是一种在能量产生中起关键作用的细胞器。细胞产生能量的能力取决于mtDNA的完整性和拷贝数。有证据表明,端粒长度和线粒体功能障碍在细胞老化和其他年龄相关疾病如癌症、糖尿病和神经退行性疾病中呈正相关。通过PPAR信号通路,两者之间可能存在机制联系。然而,具体的机理还有待阐明。ScienCell's绝对人类端粒长度和线粒体DNA拷贝数双定量qPCR试剂盒(AHDQ)被设计用于同时量化使用qPCR的人类细胞群体的平均端粒长度和mtDNA拷贝数。端粒引物集识别并扩增端粒序列。mtDNA引物集识别并扩增人类mtDNA上最保守的区域之一,不会扩增核基因组DNA上的任何脱靶序列。单拷贝参考(single copy reference, SCR)引物集识别并扩增人类17号染色体上100 bp长的区域,作为数据归一化的参考。已知端粒长度和mtDNA拷贝数的参考基因组DNA样本,作为计算目标样本端粒长度和mtDNA拷贝数的参考。精心设计的引物保证了:(1)高效可靠的定量;(ii)无非特异性扩增。每个引物通过qPCR、熔体曲线分析和凝胶电泳验证扩增特异性,模板序列稀释验证扩增效率。


Kit Components:

Cat # Component Quantity Storage
MB6018a-1 2X GoldNStart TaqGreen qPCR master mix, 1 mL 3 vials -20°C
8958a Telomere primer set, lyophilized 1 vial -20°C
8958b Human mtDNA primer set, lyophilized 1 vial -20°C
8958c Human single copy reference (SCR) primer set, lyophilized 1 vial -20°C
8958d Nuclease-free H2O 6 mL 4 °C
8958e Reference Human genomic DNA sample (Lot #30209, telomere length: 340 ± 18 kb per diploid cell;
mtDNA copy number: (1.05 ± 0.05) x 10^3 copies per diploid cell)
100 μL -20°C


货号

8958

产地

美国

缩写

AHDQ

规格

100 reactions

用途

科研

保存

液氮

运输

产品是用干冰装运的。

1.) Martin LF, Richardson LS, da Silva MG, Sheller- Miller S, Menon R. (2019) Dexamethasone Induces Primary Amnion Epithelial Cell Senescence through Telomere-P21 Associated Pathway. Biology of Reproduction.
2.) Bruno S, Sanchez MBH, Pasquino C, Tapparo M, Cedrino M, Tetta C, Camussi G. (2019) "Human Liver-Derived Stem Cells Improve Fibrosis and Inflammation Associated with Nonalcoholic Steatohepatitis." Stem Cells International. 2019
3.) Wysoczanska B, Dratwa M, Gebura K, Mizgala J, Mazur G, Wrobel T, Bogunia-Kubik K. (2019) "Variability Within the Human TERT Gene, Telomere Length and Predisposition to Chronic Lymphocytic Leukemia." OncoTargets and Therapy. 12:4309-4320
4.) Chung SS, Dutta P, Chard N, Wu Y, Chen QH, Chen G, Vadgama J. (2019) "A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-kB inactivation in colorectal cancer cells." Oncotarget. 10(44):4516-4531
5.) Babu H, Ambikan AT, Gabriel EE, Akusjarvi SS, Mupanni NR, Sperk M, Cheedarla N, Palaniapan AN, Sridhar R, Sundaraj V, Tripathy SP, Nowak P, Hanna LE, Neogi U. (2018) Systemic inflammation following long-term successful antiretroviral therapy in people living with HIV (PLHIV). bioRxiv preprint.
6.) Iezzi I, Cerqueni G, Licini c, Lucarini G, Belmonte M. (2018) Dental pulp stem cells senescence and regenerative potential relationship. Journal of Cellular Physiology. 234(5):7186-7197
7.) Lazzarini R, Nicolai M, Pirani V, Mariorri C, DiPrimio R. (2018) Effects of senescent secretory phenotype acquisition on human retinal pigment epithelial stem cells. Aging. 10(11):3173-3184
8.) Shirato K, Takanari J, Koda T, Sakurai T, Ogasawara J, Ohno H, Kizaki T. (2018) A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study. Environmental Health and Preventative Medicine. 23:40

ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.


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