产品名称	KYSE-30 人食管鳞癌细胞
货号	ZQ0963
产品介绍	这株细胞来源于治疗前从一名64岁日本男性胸中段食管切除的高分化侵袭性食管鳞状细胞癌；将肿瘤细胞异种移植到无胸腺小鼠中建立细胞系；在文献中描述为可在无胸腺小鼠中异源移植，并携带p53突变和cERB、MYC和CYCLIN D1的扩增。作为KYSE系列的一部分，该细胞系的建立是为了研究食管癌的分子和细胞特征。该细胞主要生长为贴壁单层，在相差显微镜下显示出典型的多边形形状和均匀的外观。它的生长模式是典型的上皮来源的癌细胞，形成紧密排列的集落，并倾向于以无序的方式堆积，反映了它们来源于肿瘤的侵袭性。 从遗传学上讲，KYSE-30在关键肿瘤抑制基因的改变上具有重要意义。该细胞系显示出p16 (INK4a)和p15 (INK4b)基因的野生型配置，但它在p16基因中携带一个显著的点突变，导致过早停止密码子，导致截断，无功能蛋白。这种突变可能导致细胞周期控制的丧失，促进癌细胞不受控制的增殖特征。然而，野生型p15基因的保留表明，p16基因的改变在KYSE-30的肿瘤发生中起着更关键的作用，这可能与关注这些基因在癌症中的差异作用的研究有关。 KYSE-30具有致瘤性，当注射到胸腺裸小鼠体内时，其能够形成肿瘤，使其成为ESCC体内研究的优秀模型。由KYSE-30细胞形成的肿瘤的组织学检查显示与原始鳞状细胞癌相似的特征，提供了疾病的忠实代表。该细胞系对肿瘤发生机制的研究、导致食管癌的遗传和表观遗传变化以及靶向治疗的发展具有宝贵的价值。
种属	人
性别/年龄	男/64岁
组织	食管鳞状上皮
疾病	食管鳞状细胞癌
细胞类型	肿瘤细胞
形态学	上皮细胞样，带有长的伪足
生长方式	贴壁
倍增时间	20.8 hours (PubMed=1728357); 22 hours (PubMed=25984343); ~30 hours (DSMZ=ACC-351)
培养基和添加剂	RPMI-1640（中乔新舟 货号：ZQ-200）＋F-12（中乔新舟 货号：ZQ-400）＋2mM L-Glutamine（中乔新舟 货号：CSP0012）+10%胎牛血清（中乔新舟 货号：ZQ0500）+1%双抗（中乔新舟   货号：CSP006）
推荐完全培养基货号	ZM0963
生物安全等级	BSL-1
STR位点信息	Amelogenin： X CSF1PO： 10 D2S1338： 23 D3S1358： 15,16 D5S818： 11 D7S820： 11,11.3 (JCRB=JCRB0188; PubMed=25877200) ：11,12 (DSMZ=ACC-351) D8S1179： 12,15 D13S317： 9 D16S539： 10,12 D18S51： 14 D19S433： 15,16 D21S11： 28 FGA： 24 Penta D： 12 Penta E： 13 TH01： 9 TPOX： 8,9 (JCRB=JCRB0188) ：9 (DSMZ=ACC-351; PubMed=25877200) vWA： 16,18 (DSMZ=ACC-351) ：16,18,19 (JCRB=JCRB0188; PubMed=25877200)
培养条件	95%空气，5%二氧化碳；37℃
抗原表达/受体表达	***
基因表达	***
保藏机构	DSMZ; ACC-351 ECACC; 94072011 JCRB; JCRB0188
供应限制	仅供科研使用

论文标题： miR-28-5p targets MTSS1 to regulate cell proliferation and apoptosis in esophageal cancer
DOI： 10.1093/abbs/gmaa059
发表时间： 2020-08-05
期刊： ACTA BIOCHIMICA ET BIOPHYSICA SINICA
影响因子： 2.836
货号： ZQ0963
产品名称： KYSE-30 cells

原文链接： https://academic.oup.com/abbs/article-abstract/52/8/842/5869218

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