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MFM-223人乳腺癌细胞 (STR鉴定)

英文名:MFM-223
货号:ZQ1000
价格:¥3800.00
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推荐组合

MFM-223人乳腺癌细胞 (STR鉴定)

¥3800.00
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MFM-223人乳腺癌细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

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细胞套餐惊爆价

¥4150 ¥4280.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

MFM-223人乳腺癌细胞

货号

ZQ1000

产品介绍

MFM-223是一种人乳腺癌细胞系,它源自一位未经治疗的绝经后乳腺癌患者的胸腔积液建立的。(肿瘤:广泛的乳腺导管癌T4 N2 M1,组织学为III级);文献中描述了细胞表达雄激素,雌激素和孕激素受体。MFM-223细胞系在研究中通常用于研究乳腺癌的生物学特性和疾病机制、探索乳腺癌进展和治疗抗性和新药物靶点等。

种属

性别/年龄

/45岁

组织

乳腺转移胸腔积液

疾病

乳腺癌

生物安全等级

BSL-1

STR位点信息

Amelogenin:X
CSF1PO:10
D13S317:8,12
D16S539:11
D5S818:11,12
D7S820:8,10
THO1:6,9.3
TPOX:8
vWA:17

细胞类型

肿瘤细胞

形态学

 上皮样

生长方式

贴壁细胞以单层生长,具有网状生长模式

倍增时间

184 hours (Note=At 12th passage), ~70 hours (Note=At 35th passage) (PubMed=1913690); ~5-7 days (DSMZ=ACC-422)

培养基和添加剂

MEM(中乔新舟 货号:ZQ-300)+10%FBS(中乔新舟 货号:ZQ500-A)+1%PS(中乔新舟 货号:CSP006

推荐完全培养基货号

ZM1000

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 文献报道:雄激素、雌激素和孕激素受体

基因表达

 ***

保藏机构

DSMZ; ACC-422  ECACC; 98050130 

供应限制

仅供科研使用


货号

ZQ1000

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用

PubMed=1913690
Hackenberg R., Luttchens S., Hofmann J., Kunzmann R., Holzel F., Schulz K.-D.
Androgen sensitivity of the new human breast cancer cell line MFM-223.
Cancer Res. 51:5722-5727(1991)


DOI=10.1016/B978-0-12-333530-2.50009-5
Leibovitz A.
Cell lines from human breast.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.161-184; Academic Press; New York (1994)


PubMed=20164919; DOI=10.1038/nature08768
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Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)


PubMed=22414580; DOI=10.1158/0008-5472.CAN-11-3711
Geiger T., Madden S.F., Gallagher W.M., Cox J., Mann M.
Proteomic portrait of human breast cancer progression identifies novel prognostic markers.
Cancer Res. 72:2428-2439(2012)


PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110
Daemen A., Griffith O.L., Heiser L.M., Wang N.J., Enache O.M., Sanborn Z., Pepin F., Durinck S., Korkola J.E., Griffith M., Hur J.S., Huh N., Chung J., Cope L., Fackler M.J., Umbricht C.B., Sukumar S., Seth P., Sukhatme V.P., Jakkula L.R., Lu Y.-L., Mills G.B., Cho R.J., Collisson E.A., van 't Veer L.J., Spellman P.T., Gray J.W.
Modeling precision treatment of breast cancer.
Genome Biol. 14:R110.1-R110.14(2013)


PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)


PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)


PubMed=25892236; DOI=10.1016/j.celrep.2015.03.050
Lawrence R.T., Perez E.M., Hernandez D., Miller C.P., Haas K.M., Irie H.Y., Lee S.-I., Blau C.A., Villen J.
The proteomic landscape of triple-negative breast cancer.
Cell Rep. 11:630-644(2015)


PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)


PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)


PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)


PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)


PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

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