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PA-1人卵巢畸胎瘤细胞(STR鉴定)

英文名:PA-1
货号:ZQ0725
价格:¥1200.00
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PA-1人卵巢畸胎瘤细胞(STR鉴定)

¥1200.00
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PA-1人卵巢畸胎瘤细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥1550 ¥1680.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

PA-1人卵巢畸胎瘤细胞

货号

ZQ0725

产品介绍

PA-1是一种人卵巢畸胎瘤细胞系,最初是从一名12岁白人女性患者的腹水(ascitic fluid)中提取的细胞建立的。这些细胞在培养时能够形成紧密相连的菌落,细胞呈现上皮细胞样形态,贴壁生长。并且在低血清浓度、低接种密度或经过5-溴-2'-脱氧尿苷(5-bromo-2'-deoxyuridine)处理的条件下,能够分化形成类胚体(embryoid bodies)。

此外,PA-1细胞表达胚胎抗原PCC4,这是一种在早期胚胎发育中出现的蛋白质,但F9抗原不可检测。PCC4的表达表明这些细胞具有胚胎干细胞的特性,而F9的不表达则可能表明它们在分化过程中已经失去了某些原始的胚胎细胞特性。这种细胞系对于研究胚胎发育、畸胎瘤的形成机制以及干细胞分化过程中的分子调控机制具有重要价值。也可广泛应用于药物筛选、基因功能研究、信号通路研究、肿瘤转移机制研究以及干细胞特性研究等领域。

种属

性别/年龄

/12岁

组织

卵巢;源自转移部位:腹水

疾病

畸胎癌

细胞类型

肿瘤细胞

形态学

上皮样

生长方式

贴壁

倍增时间

36 hours (PubMed=2653399); ~24 hours (Note=Lot 01212002) (JCRB=JCRB9061); 22.77 hours (JWGray panel)

培养基和添加剂

MEM(含NEAA)培养基(中乔新舟  货号:ZQ-300)+10%胎牛血清(中乔新舟  货号:ZQ500-A+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZQ-301

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;37℃

STR位点信息

Amelogenin: X

CSF1PO: 9,12

D13S317: 9,10

D16S539: 9,12

D5S818: 11

D7S820: 9

THO1: 7,9

TPOX: 11

vWA: 15,17

抗原表达/受体表达

*** 

基因表达

*** 

保藏机构

ATCC;CRL-1572;BCRC;60063ECACC;90013101JCRB;JCRB9061

供应限制

仅供科研使用


货号

ZQ0725

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用

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An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells.
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Hills C.A., Kelland L.R., Abel G., Siracky J., Wilson A.P., Harrap K.R.
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PubMed=11314036; DOI=10.1038/sj.onc.1204211
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The human ovarian teratocarcinoma cell line PA-1 demonstrates a single translocation: analysis with fluorescence in situ hybridization, spectral karyotyping, and bacterial artificial chromosome microarray.
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PubMed=20164919; DOI=10.1038/nature08768
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Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
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