产品名称 |
SNU-16人胃癌细胞 |
货号 |
ZQ0747 |
产品介绍 |
SNU-16是一种人类胃癌细胞系,是1987年由J. Park及其同事从一位33岁亚洲女性胃癌患者化疗前的腹水中分离出来,该细胞系是由化疗前提取的细胞建立的。这些细胞具有上皮形态,并且是悬浮聚团生长的。SNU-16细胞表达表面糖蛋白癌胚抗原(CEA)和TAG-72,L-多巴脱羧酶(DDC)阳性。VIP受体阳性,但胃受体缺失。c-myc原癌基因存在扩增,但表达的c-erb-B-2 RNA水平与其他细胞株相当。该细胞不表达N-myc,L-myc,c-cis,IGF-2及胃泌素释放肽。这些细胞在癌症研究中非常有用,尤其是在研究胃癌的生物学行为、药物反应和信号传导途径方面。它们也可以用于测试新的抗癌药物和治疗方法。 |
种属 |
人 |
性别/年龄 |
女/33岁 |
组织 |
胃 |
疾病 |
胃癌 |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮样 |
生长方式 |
悬浮,多细胞聚集体 |
倍增时间 |
27 hours (ATCC=CRL-5974; PubMed=2158397; PubMed=29435981) |
培养基和添加剂 |
RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
STR位点信息 |
Amelogenin: X
D3S1358: 15,16
|
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-5974;BCRC; 60212;KCLB; 00016 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0747 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=2158397
Park J.-G., Frucht H., La Rocca R.V., Bliss D.P. Jr., Kurita Y., Chen T.-R., Henslee J.G., Trepel J.B., Jensen R.T., Johnson B.E., Bang Y.-J., Kim J.-P., Gazdar A.F.
Characteristics of cell lines established from human gastric carcinoma.
Cancer Res. 50:2773-2780(1990)
PubMed=1676761; DOI=10.1093/jnci/83.13.938
Kim J.-H., Takahashi T., Chiba I., Park J.-G., Birrer M.J., Roh J.K., De Lee H., Kim J.-P., Minna J.D., Gazdar A.F.
Occurrence of p53 gene abnormalities in gastric carcinoma tumors and cell lines.
J. Natl. Cancer Inst. 83:938-943(1991)
DOI=10.1016/B978-0-12-333530-2.50014-9
Sekiguchi M., Suzuki T.
Gastric tumor cell lines.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.287-316; Academic Press; New York (1994)
PubMed=8806092; DOI=10.1002/jcb.240630505
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NCI-Navy Medical Oncology Branch cell line data base.
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Park J.-G., Gazdar A.F.
Biology of colorectal and gastric cancer cell lines.
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PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3
Ku J.-L., Yoon K.-A., Kim D.-Y., Park J.-G.
Mutations in hMSH6 alone are not sufficient to cause the microsatellite instability in colorectal cancer cell lines.
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Kim H.-P., Lee M.-S., Yu J., Park J.-A., Jong H.-S., Kim T.-Y., Lee J.W., Bang Y.-J.
TGF-beta1 (transforming growth factor-beta1)-mediated adhesion of gastric carcinoma cells involves a decrease in Ras/ERKs (extracellular-signal-regulated kinases) cascade activity dependent on c-Src activity.
Biochem. J. 379:141-150(2004)
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Ku J.-L., Park J.-G.
Biology of SNU cell lines.
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Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)
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A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
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The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)
PubMed=23637631; DOI=10.1371/journal.pgen.1003464
Giacomini C.P., Sun S., Varma S., Shain A.H., Giacomini M.M., Balagtas J.M.S., Sweeney R.T., Lai E., Del Vecchio C.A., Forster A.D., Clarke N., Montgomery K.D., Zhu S., Wong A.J., van de Rijn M., West R.B., Pollack J.R.
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PLoS Genet. 9:E1003464-E1003464(2013)
PubMed=24807215; DOI=10.1038/ncomms4830
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Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.
Nat. Commun. 5:3830.1-3830.8(2014)
PubMed=25960936; DOI=10.4161/21624011.2014.954893
Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.
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OncoImmunology 3:e954893.1-e954893.12(2014)
PubMed=25485619; DOI=10.1038/nbt.3080
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A comprehensive transcriptional portrait of human cancer cell lines.
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A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)
PubMed=26589293; DOI=10.1186/s13073-015-0240-5
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A landscape of pharmacogenomic interactions in cancer.
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Forty-nine gastric cancer cell lines with integrative genomic profiling for development of c-MET inhibitor.
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An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
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