Description |
Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable . The biologically active native form of TNF-? is reportedly a trimer. Two types of receptors for TNF-? have been described and virtually all cell types studied show the presence of one or both of these receptor types. |
Synonyms |
TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFa, Cachectin, DIF, TNFA, TNFSF2 |
AA Sequence |
MLRSSSQNSS DKPVVHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLVVPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSPCPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQVYFGVIAL |
Source |
Escherichia coli |
Molecular Weight |
Approximately 17.3 kDa. a single, non-glycosylated polypeptide chain containing 157 amino acids. |
Biological Activity |
Fully biologically active. Specific activity ? 5 |
Physical Appearance |
White lyophilized powder. |
Formulation |
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.2, 150mM NaCl. |
Endotoxin |
< 1EU/μg of growth factor as determined by LAL method. |
Reconstitution |
Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |
Storage |
Store at -20°C after receiving. Upon Reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles. |
Usage |
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
货号 | 143-01 |
产地 | 美国 |
缩写 |
rrTNF-α |
规格 |
5µg |
用途 | 科研 |
包装 | 管装 |
保存 |
-20℃冰箱 |