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名称 |
MC3T3-E1 subclone 14小鼠胚胎成骨细胞 |
货号 |
ZQ0095 |
产品介绍 |
从克隆的但是表型各异的MC3T3-E1细胞系中分离出一系列亚克隆。 从含抗坏血酸培养基生长的成骨细胞中选择高或低成骨细胞分化、矿化的亚克隆。 MC3T3亚克隆4(ATCC CRL-2593)和MC3T3亚克隆14(ATCC CRL-2594)在抗坏血酸和3到4mM无机磷酸盐中生长表现出高水平的成骨细胞分化。 它们10天后形成一个矿化良好的细胞外基质(ECM)。 MC3T3亚克隆24(ATCC CRL-2595)和MC3T3亚克隆30(ATCC CRL-2596)在抗坏血酸中生长表现出很差的成骨细胞分化。 不形成ECM, 可以作为亚克隆4和14的阴性对照。 矿化的亚克隆选择的表达作为成骨细胞标记的mRNA,及唾液酸糖蛋白(BSP),骨钙素(OCN),和甲状旁腺激素/甲状旁腺激素相关蛋白受体的mRNA。 高或者低的分化潜能的亚克隆在培养中生产出相似数量的胶原质,表达可比较的基本水平的mRNA编码Osf2/Cbfa1,一种成骨细胞相关转录因子。 植入免疫缺陷小鼠以后,高分化性的亚克隆形成与骨类似的形成小骨的编织骨,低分化细胞只是产生纤维组织。 这些细胞系是研究体外成骨细胞分化的好模型,尤其是ECM信号。 它们和原代培养颅顶成骨细胞的行为类似。 注意:MEM α 不含VC组分 |
种属 |
小鼠 |
组织来源 |
骨骼、头盖组织 |
形态学 |
成纤维细胞样 |
细胞类型 |
自然永生细胞系 |
倍增时间 |
~1-2 days (DSMZ=ACC-210) |
生长方式 |
贴壁 |
培养基和添加剂 |
MEM α(中乔新舟 货号:ZQ-700-VC-FREE)+10%胎牛血清(中乔新舟 货号:ZQ0500) +1%P/S(中乔新舟 货号:CSP006) 注意:MEM α 不含VC组分 |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
ATCC; CRL-2953 CCTCC; GDC0188 DSMZ; ACC-210 ECACC; 99072810 RCB; RCB1126 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0095 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
1.Inhibiting Osteolytic Breast Cancer Bone Metastasis by Bone-Targeted Nanoagent via Remodeling the Bone Tumor Microenvironment Combined with NIR-II Photothermal Therapy
作者:Yaoxun Zeng, Zhenxing Pan, Jiongpeng Yuan, Yuqiong Song, Zhenzhen Feng, Zefeng Chen, Zhaoyi Ye, Yushan Li, Ying Bao, Zhili Ran, Xinyi Li, Huiling Ye, Kun Zhang, Xujie Liu, Yan He
期刊:Small
DOI:First published: 21 May 2023 https://doi.org/10.1002/smll.202301003
影响因子 :15.153
引用产品:ZQ0118,ZQ0095,ZQ0098
2.Amino-Functionalized Zirconium-Based Metal–Organic Frameworks as Bifunctional Nanomaterials to Treat Bone Tumors and Promote Osteogenesis.(2023-11-09)
作者:Jongpeng Yuan,Xujie Liu
期刊:ACS Applied Materials & Interfaces
DOI: 10.1021/acsami.3c11787
影响因子 :9.5
引用产品:MC3T3-E1, MNNG/HOS and MDA-MB-231 cell
https://pubs.acs.org/doi/full/10.1021/acsami.3c11787
3.Chitosan/NH2-MIL-125 (Ti) scaffold loaded with doxorubicin for postoperative bone tumor clearance and osteogenesis: An in vitro study.( Pub Date : 2024-2-23)
作者:Yaoxun Zeng , Jiongpeng Yuan
期刊:International Journal of Biological Macromolecules
DOI: DOI:10.1016/j.ijbiomac.2024.130368
影响因子 :8.2
引用产品: (MC3T3-E1 cells, ZQ0095),(RAW264.7 cells, ZQ0098),(MDA-MB-231 cells, ZQ0118),(HUVEC cells, ZQY004)
https://www.sciencedirect.com/science/article/pii/S0141813024011711?via%3Dihub
4.论文标题: Self-Organized Spatiotemporal Mineralization of Hydrogel: A Simulant of Osteon
DOI: 10.1002/smll.202106649
发表时间: 2021-12-18
期刊: Small
影响因子: 13.281
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.202106649
5.论文标题: A Stable Cell Membrane-Based Coating with Antibiofouling and Macrophage Immunoregulatory Properties for Implants at the Macroscopic Level
DOI: 10.1021/acs.chemmater.1c01957
发表时间: 2021-10-14
期刊: CHEMISTRY OF MATERIALS
影响因子: 9.811
货号: ZQ0095
产品名称: MC3T3 subclone line
原文链接: https://pubs.acs.org/doi/abs/10.1021/acs.chemmater.1c01957
6.论文标题: Wood-Derived Hybrid Scaffold with Highly Anisotropic Features on Mechanics and Liquid Transport toward Cell Migration and Alignment
DOI: 10.1021/acsami.0c00646
发表时间: 2020-03-20
期刊: ACS Applied Materials & Interfaces
影响因子: 8.758
货号: ZQ0095
产品名称: MC3T3-E1 subclone cell line
原文链接: https://pubs.acs.org/doi/abs/10.1021/acsami.0c00646
7.论文标题: Biomimetic bone regeneration using angle-ply collagen membrane-supported cell sheets subjected to mechanical conditioning
DOI: 10.1016/j.actbio.2020.05.041
发表时间: 2020-06-04
期刊: Acta Biomaterialia
影响因子: 7.242
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S1742706120303202
8.论文标题: Evaluation of anti-tumor effects of crocin on a novel 3D tissue-engineered tumor model based on sodium alginate/gelatin microbead
DOI: 10.1016/j.ijbiomac.2021.01.181
发表时间: 2021-01-30
期刊: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
影响因子: 6.953
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0141813021002294
9.论文标题: Silk fibroin hydrogels induced and reinforced by acidic calcium phosphate – A simple way of producing bioactive and drug-loadable composites for biomedical applications
DOI: 10.1016/j.ijbiomac.2021.10.160
发表时间: 2021-10-26
期刊: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
影响因子: 6.953
货号: ZQ0095
产品名称: MC3T3 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S014181302102314X
10.论文标题: Dual crosslinking hydrogels with tunable injectability and stability for bone repair
DOI: 10.1039/D2TB00545J
发表时间: 2022-04-21
期刊: Journal of Materials Chemistry B
影响因子: 6.331
货号: ZQ0095
产品名称: mouse MC3T3-E1 sub-clone cell line
原文链接: https://pubs.rsc.org/en/content/articlelanding/2022/tb/d2tb00545j
11.论文标题: Microfluidic Fabrication of Biomimetic Helical Hydrogel Microfibers for Blood-Vessel-on-a-Chip Applications
DOI: 10.1002/adhm.201900435
发表时间: 2019-05-13
期刊: Advanced Healthcare Materials
影响因子: 6.27
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.201900435
12.论文标题: A natural polymer based bioadhesive with self-healing behavior and improved antibacterial properties
DOI: 10.1039/D0BM00624F
发表时间: 2020-06-19
期刊: Biomaterials Science
影响因子: 6.183
货号: ZQ0095
产品名称: MC3T3-E1 sub-clone cell line
原文链接: https://pubs.rsc.org/en/content/articlelanding/2020/bm/d0bm00624f
13.论文标题: Ginsenoside Rc Promotes Bone Formation in Ovariectomy-Induced Osteoporosis In Vivo and Osteogenic Differentiation In Vitro
DOI: 10.3390/ijms23116187
发表时间: 2022-05-31
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子: 5.924
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://www.mdpi.com/1422-0067/23/11/6187
14.论文标题: A mussel-bioinspired multi-functional hyperbranched polymeric coating with integrated antibacterial and antifouling activities for implant interface modification
DOI: 10.1039/D1PY00246E
发表时间: 2021-05-10
期刊: Polymer Chemistry
影响因子: 5.582
货号: ZQ0095
产品名称: mouse MC3T3(-E1) subclonal cell line
原文链接: https://pubs.rsc.org/en/content/articlelanding/2021/xx/d1py00246e
15.论文标题: Crosstalk between the COX2‐PGE2‐EP4 signaling pathway and primary cilia in osteoblasts after mechanical stimulation
DOI: 10.1002/jcp.30198
发表时间: 2020-12-04
期刊: JOURNAL OF CELLULAR PHYSIOLOGY
影响因子: 5.546
货号: ZQ0095
产品名称: MC3T3‐E1 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.30198
16.论文标题: A facile strategy to construct silk fibroin based GTR membranes with appropriate mechanical performance and enhanced osteogenic capacity
DOI: 10.1039/D0TB01962C
发表时间: 2020-10-09
期刊: Journal of Materials Chemistry B
影响因子: 5.344
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://pubs.rsc.org/en/content/articlelanding/2020/tb/d0tb01962c
17.论文标题: Bioinspired by both mussel foot protein and bone sialoprotein: universal adhesive coatings for the promotion of mineralization and osteogenic differentiation
DOI: 10.1039/D0PY00774A
发表时间: 2020-06-29
期刊: Polymer Chemistry
影响因子: 5.342
货号: ZQ0095
产品名称: MC3T3-E1 subclone cell line
原文链接: https://pubs.rsc.org/en/content/articlelanding/2020/py/d0py00774a
18.论文标题: Promotion of the osteogenic activity of an antibacterial polyaniline coating by electrical stimulation
DOI: 10.1039/C9BM01203F
发表时间: 2019-08-26
期刊: Biomaterials Science
影响因子: 5.251
货号: ZQ0095
产品名称: MCT3T3-E1 cell line
原文链接: https://pubs.rsc.org/en/content/articlelanding/2019/bm/c9bm01203f
19.论文标题: Exosomes from bone marrow mesenchymal stem cells enhance fracture healing through the promotion of osteogenesis and angiogenesis in a rat model of nonunion
DOI: 10.1186/s13287-020-1562-9
发表时间: 2020-01-28
期刊: Stem Cell Research & Therapy
影响因子: 5.116
货号: ZQ0095
产品名称: MC3T3-E1Cs
原文链接: https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-1562-9
20.论文标题: Supramolecular nanoassemblies of salmon calcitonin and aspartame for fibrillation inhibition and osteogenesis improvement
DOI: 10.1016/j.ijpharm.2020.120171
发表时间: 2020-12-13
期刊: INTERNATIONAL JOURNAL OF PHARMACEUTICS
影响因子: 4.845
货号: ZQ0095
产品名称: MC3T3-E1 subclonal cells
原文链接: https://www.sciencedirect.com/science/article/pii/S037851732031156X
21.论文标题: Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis
DOI: 10.3390/biom9050173
发表时间: 2019-05-05
期刊: Biomolecules
影响因子: 4.694
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://www.mdpi.com/456906
22.论文标题: Evaluation of Differentiated Bone Cells Proliferation by Blue Shark Skin Collagen via Biochemical for Bone Tissue Engineering
DOI: 10.3390/md16100350
发表时间: 2018-09-25
期刊: Marine Drugs
影响因子: 4.379
货号: ZQ0095
产品名称: MC3T3E1 cells
原文链接: https://www.mdpi.com/343572
23.论文标题: Direct Current Stimulation for Improved Osteogenesis of MC3T3 Cells Using Mineralized Conductive Polyaniline
DOI: 10.1021/acsbiomaterials.9b01821
发表时间: 2020-03-16
期刊: ACS Biomaterials Science & Engineering
影响因子: 4.152
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.9b01821
24.论文标题: Primary cilia respond to intermittent low-magnitude, high-frequency vibration and mediate vibration-induced effects in osteoblasts
DOI: 10.1152/ajpcell.00273.2019
发表时间: 2019-12-18
期刊: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
影响因子: 3.553
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://journals.physiology.org/doi/abs/10.1152/ajpcell.00273.2019
25.论文标题: miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16
DOI: 10.1186/s12935-019-0829-7
发表时间: 2019-04-23
期刊: Cancer Cell International
影响因子: 3.439
货号: ZQ0095
产品名称: MC3T3 cells
原文链接: https://cancerci.biomedcentral.com/articles/10.1186/s12935-019-0829-7
26.论文标题: Microfluidics-Based Fabrication of Cell-Laden Hydrogel Microfibers for Potential Applications in Tissue Engineering
DOI: 10.3390/molecules24081633
发表时间: 2019-04-25
期刊: MOLECULES
影响因子: 3.06
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://www.mdpi.com/451774
27.论文标题: Effect of GelMA Hydrogel Coatings on Corrosion Resistance and Biocompatibility of MAO-Coated Mg Alloys
DOI: 10.3390/ma13173834
发表时间: 2020-08-30
期刊: Materials
影响因子: 3.057
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://www.mdpi.com/812528
28.论文标题: Overexpression of fucosyltransferase 8 reverses the inhibitory effect of high-dose dexamethasone on osteogenic response of MC3T3-E1 preosteoblasts
DOI: 10.7717/peerj.12380
发表时间: 2021-12-09
期刊: PeerJ
影响因子: 2.984
货号: ZQ0095
产品名称: Murine osteoblast precursor cell line MC3T3-E1
原文链接: https://peerj.com/articles/12380/
29.论文标题: Protective effects of low-magnitude high-frequency vibration on high glucose-induced osteoblast dysfunction and bone loss in diabetic rats
DOI: 10.1186/s13018-021-02803-w
发表时间: 2021-10-30
期刊: Journal of Orthopaedic Surgery and Research
影响因子: 2.359
货号: ZQ0095
产品名称: MC3T3-E1 cells
原文链接: https://link.springer.com/article/10.1186/s13018-021-02803-w
30.论文标题: Ginsenoside Rg3 attenuates ovariectomy‐induced osteoporosis via AMPK/mTOR signaling pathway
DOI: 10.1002/ddr.21705
发表时间: 2020-06-17
期刊: DRUG DEVELOPMENT RESEARCH
影响因子: 1.902
货号: ZQ0095
产品名称: osteoblastic cell line MC3T3-E1
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/ddr.21705
31.论文标题: Duration of electrochemical deposition affects the morphology of hydroxyapatite coatings on 3D-printed titanium scaffold as well as the functions of adhered MC3T3-E1 cells
DOI: 10.1016/j.jos.2019.09.008
发表时间: 2019-10-10
期刊: JOURNAL OF ORTHOPAEDIC SCIENCE
影响因子: 1.257
货号: ZQ0095
产品名称: MC3T3-E1 line
原文链接: https://www.sciencedirect.com/science/article/pii/S0949265819302829