名称 |
Vero非洲绿猴肾细胞(种属鉴定)(种属鉴定) |
货号 |
ZQ0159 |
产品介绍 |
Vero细胞株是日本千叶大学的Y. Yasumura和Y. Kawakita从正常成年非洲绿猴的肾脏建株的。1964年6月15日B. Simizu将其从千叶大学带到国立健康研究所(NIH)国立过敏及传染病研究所热带病毒实验室时,已传至第93代。 |
种属 |
非洲绿猴 |
组织来源 |
原位;肾 |
形态学 |
上皮样 |
细胞类型 |
自发永生化细胞系 |
倍增时间 |
|
生长方式 |
贴壁 |
培养基和添加剂 |
DMEM高糖(品牌:中乔新舟 货号:ZQ-100)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%双抗(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
AddexBio; P0014002/4920 ATCC; CCL-81 ATCC; CCL-81.4 BCRC; 60013 BCRJ; 0245 CCLV; CCLV-RIE 0015 CCLV; CCLV-RIE 0078 CCTCC; GDC0029 CLS; 605372 ECACC; 08011101 ECACC; 84113001 IBRC; C10001 ICLC; ATL95005 IZSLER; BS CL 86 JCRB; IFO50471 JCRB; JCRB0111 JCRB; JCRB9013 KCB; KCB 92017YJ KCLB; 10081 NCBI_Iran; C101 RCB; RCB0001 TKG; TKG 0280 |
供应限制 |
仅供科研使用 |
货号 |
ZM159 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
CLPUB00126
Yasumura Y., Kawakita Y.
Studies on SV40 in tissue culture: preliminary step for cancer research in vitro.
Nihon Rinsho 21:1201-1219(1963)
PubMed=6027511; DOI=10.3181/00379727-125-32029
Simizu B., Rhim J.S., Wiebenga N.H.
Characterization of the Tacaribe group of arboviruses. I. Propagation and plaque assay of Tacaribe virus in a line of African green monkey kidney cells (Vero).
Proc. Soc. Exp. Biol. Med. 125:119-123(1967)
DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)
PubMed=6298990; DOI=10.1016/0378-1135(82)90056-6
McPhee D.A., Parsonson I.M., Della-Porta A.J.
Comparative studies on the growth of Australian bluetongue virus serotypes in continuous cell lines and embryonated chicken eggs.
Vet. Microbiol. 7:401-410(1982)
PubMed=4043530
Whitaker A.M., Hayward C.J.
The characterization of three monkey kidney cell lines.
Dev. Biol. Stand. 60:125-131(1985)
CLPUB00328
Earley E.M., Johnson K.M.
The lineage of Vero, Vero 76 and its clone C1008 in the United States.
(In) Vero cells: origin, properties and biomedical applications; Simizu B., Terasima T. (eds.); pp.26-29; Department of Microbiology School of Medicine Chiba University; Chiba (1988)
PubMed=2260226
Tsareva A.A., Isaenko A.A., Urmanova M.A., Yurchenko N.D., Balzovskaia E.G., Rodionova O.M.
A karyotype study of the Vero cell line cultured long term in a monolayer by static and roller methods.
Tsitologiia 32:741-748(1990)
PubMed=10403033; DOI=10.1006/biol.1998.0156
Lang J., Cetre J.C., Picot N., Lanta M., Briantais P., Vital S., Le Mener V., Lutsch C., Rotivel Y.
Immunogenicity and safety in adults of a new chromatographically purified Vero-cell rabies vaccine (CPRV): a randomized, double-blind trial with purified Vero-cell rabies vaccine (PVRV).
Biologicals 26:299-308(1998)
PubMed=10494966
Montagnon B.J., Vincent-Falquet J.-C., Saluzzo J.-F.
Experience with Vero cells at Pasteur Merieux Connaught.
Dev. Biol. Stand. 98:137-140(1999)
PubMed=12667817; DOI=10.1016/S0042-6822(02)00064-8
Romanova J., Katinger D., Ferko B., Voglauer R., Mochalova L., Bovin N., Lim W., Katinger H., Egorov A.
Distinct host range of influenza H3N2 virus isolates in Vero and MDCK cells is determined by cell specific glycosylation pattern.
Virology 307:90-97(2003)
PubMed=15731278; DOI=10.1128/JVI.79.6.3846-3850.2005
Mossel E.C., Huang C., Narayanan K., Makino S., Tesh R.B., Peters C.J.
Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.
J. Virol. 79:3846-3850(2005)
PubMed=16494729; DOI=10.3201/eid1201.050496
Kaye M., Druce J., Tran T., Kostecki R., Chibo D., Morris J., Catton M., Birch C.
SARS-associated coronavirus replication in cell lines.
Emerg. Infect. Dis. 12:128-133(2006)
DOI=10.12665/J63.Morgan
Morgan J.H.
Vero cells in vaccine production.
BioProcess. J. 6:12-17(2007)
PubMed=19016439; DOI=10.1002/9780471729259.mca04es11
Ammerman N.C., Beier-Sexton M., Azad A.F.
Growth and maintenance of Vero cell lines.
Curr. Protoc. Microbiol. 11:A.4E.1-A.4E.7(2008)
PubMed=19768803; DOI=10.1002/btpr.279
Rourou S., van der Ark A., van der Velden T., Kallel H.
Development of an animal-component free medium for Vero cells culture.
Biotechnol. Prog. 25:1752-1761(2009)
PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)
PubMed=22059503; DOI=10.1186/1472-6750-11-102
Almeida J.L., Hill C.R., Cole K.D.
Authentication of African green monkey cell lines using human short tandem repeat markers.
BMC Biotechnol. 11:102.1-102.10(2011)
PubMed=24975811; DOI=10.1016/j.vaccine.2014.06.045
Donis R.O., Chen L.-M., Davis C.T., Foust A., Hossain M.J., Johnson A., Klimov A., Loughlin R.F., Xu X.-Y., Tsai T., Blayer S., Trusheim H., Colegate T., Fox J., Taylor B., Hussain A.I., Barr I., Baas C., Louwerens J., Geuns E., Lee M.-S., Venhuizen L., Neumeier E., Ziegler T.
Performance characteristics of qualified cell lines for isolation and propagation of influenza viruses for vaccine manufacturing.
Vaccine 32:6583-6590(2014)
PubMed=25267831; DOI=10.1093/dnares/dsu029
Osada N., Kohara A., Yamaji T., Hirayama N., Kasai F., Sekizuka T., Kuroda M., Hanada K.
The genome landscape of the African green monkey kidney-derived Vero cell line.
DNA Res. 21:673-683(2014)
PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)
PubMed=28386771; DOI=10.1007/s10616-017-0082-7
Andreani N.A., Renzi S., Piovani G., Ajmone-Marsan P., Bomba L., Villa R., Ferrari M., Dotti S.
Potential neoplastic evolution of Vero cells: in vivo and in vitro characterization.
Cytotechnology 69:741-750(2017)
PubMed=32376603; DOI=10.1126/science.abc1932
Gao Q., Bao L.-L., Mao H.-Y., Wang L., Xu K.-W., Yang M.-N., Li Y.-J., Zhu L., Wang N., Lv Z., Gao H., Ge X.-Q., Kan B., Hu Y.-L., Liu J.-N., Cai F., Jiang D.-Y., Yin Y.-H., Qin C.-F., Li J., Gong X.-J., Lou X.-Y., Shi W., Wu D.-D., Zhang H.-M., Zhu L., Deng W., Li Y.-R., Lu J.-X., Li C.-G., Wang X.-X., Yin W.-D., Zhang Y.-J., Qin C.
Development of an inactivated vaccine candidate for SARS-CoV-2.
Science 369:77-81(2020)
PubMed=32511316; DOI=10.1101/2020.03.02.972935
Harcourt J.L., Tamin A., Lu X.-Y., Kamili S., Kumar-Sakthivel S., Murray J., Queen K., Tao Y., Paden C.R., Zhang J., Li Y., Uehara A., Wang H.-B., Goldsmith C., Bullock H.A., Wang L.-J., Whitaker B., Lynch B., Gautam R., Schindewolf C., Lokugamage K.G., Scharton D., Plante J.A., Mirchandani D., Widen S.G., Narayanan K., Makino S., Ksiazek T.G., Plante K.S., Weaver S.C., Lindstrom S., Tong S.-X., Menachery V.D., Thornburg N.J.
Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient.
bioRxiv 2020:972935-972935(2020)
PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)
货号 | 产品名称 | 规格 | 价格 | 指令 |
ZQ0156 | COS-1非洲绿猴SV40转化的肾细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1200.00 | 放入购物车 》 |
ZQ0157 | COS-7非洲绿猴SV40转化的肾细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1000.00 | 放入购物车 》 |
ZQ0158 | CV-1[Part of the Wistar Special Collection]非洲绿猴肾细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1000.00 | 放入购物车 》 |
ZQ0160 | VERO C1008 (E6)非洲绿猴肾细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ0161 | FRhK-4恒河猴胚肾细胞 | 5 x 10^5 cells/vial | ¥1200.00 | 放入购物车 》 |
ZQ0162 | LLC-MK2恒河猴肾细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1000.00 | 放入购物车 》 |
ZQ0175 | Pt K1 (NBL-3)袋鼠肾细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1200.00 | 放入购物车 》 |
ZQ506 | 支原体清除试剂盒 | 1ml | ¥600.00 | 放入购物车 》 |
A1001-1 | 国产转染试剂 | 1ml | ¥800.00 | 放入购物车 》 |
1 | 慢病毒介导基因沉默或过表达 | ¥询价 | 放入购物车 》 | |
6 | 绿/红色荧光蛋白标记 | ¥询价 | 放入购物车 》 | |
ZQ-100 | DMEM(高糖)基础培养基 | 500ml | ¥56.00 | 放入购物车 》 |
ZQ-101 | DMEM(高糖)完全培养基 | 500ML | ¥350.00 | 放入购物车 》 |
ZQ500-A | 优级胎牛血清 | 500ml | ¥2580(开学促销) | 放入购物车 》 |
JSFW-134 | 人源细胞STR鉴定服务 | ¥900.00 | 放入购物车 》 |