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HCT-15人结直肠腺癌细胞(STR鉴定)

英文名:HCT-15
货号:ZQ0324
价格:¥1500.00
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推荐组合

HCT-15人结直肠腺癌细胞(STR鉴定)

¥1500.00
+

HCT-15人结直肠腺癌细胞专用培养基

¥350.00 ¥480.00

配套完培,组合使用,省时省力

=

细胞套餐惊爆价

¥1850 ¥1980.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

HCT-15人结直肠腺癌细胞

货号

ZQ0324

产品介绍

HCT-15细胞来源于一名男性的结肠腺癌。该细胞系于20世纪70年代初被发现,广泛应用于癌症研究领域,特别是探索结直肠癌的生物学和治疗。DNA fingerprinting证据表明,这株细胞和DLD-1 (ATCC CCL-221) 来源于同一个人;但同工酶及细胞染色体组型分析仍存疑问。 细胞呈CSAp阴性(CSAp-)。 角蛋白免疫过氧化物酶染色阳性。
在形态学上,HCT-15细胞的特征是上皮样外观,倾向于单层和簇状生长,表现出明显的细胞异质性。这一特征反映了实体肿瘤中发现的不同细胞环境,使HCT-15成为研究肿瘤动力学和肿瘤微环境内细胞相互作用的有价值模型。
基因型上,HCT-15细胞表现为多染色体畸变的超二倍体核型,这是许多结直肠癌的典型特征。这些突变包括关键癌基因和肿瘤抑制基因的突变,如KRAS基因的突变和影响p53通路的缺失,这些突变与结直肠癌的发病和进展有关。这些遗传特征使HCT-15细胞成为研究与癌症进展、转移和治疗耐药性相关的遗传和分子机制的重要工具。
HCT-15细胞在研究中的广泛应用使我们对结肠直肠癌的分子通路有了重要的了解,增强了我们对疾病机制的理解,并有助于开发靶向治疗。

注意事项:
HCT-15细胞块状生长,较难消化可适当延长消化时间。

种属

性别/年龄

组织

结肠

疾病

杜克斯氏C型,结肠直肠腺癌

细胞类型

肿瘤细胞 

形态学

上皮

生长方式

贴壁

倍增时间

 大约19~29.57小时

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟  货号:ZQ500-A+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0324

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y
D5S818:13
D13S317: 8,11
D7S820: 10,12
D16S539: 12,13
vWA: 18,19
TH01: 7,9.3
TPOX: 8,11
CSF1PO: 12
D19S433: 14,16
D21S11: 29,32.2
D18S51: 11,17 

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

  ***

基因表达

  ***

保藏机构

ATCC; CCL-225 BCRC; 60539 DSMZ; ACC-357 ECACC; 91030712

供应限制

仅供科研使用

货号

ZQ0324

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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