产品名称	T84人结肠腺癌肺转移细胞
货号	ZQ0328
产品介绍	T84细胞株是从一位72岁男性结肠癌患者的肺转移灶建立的可移植人类癌细胞株。 肿瘤组织皮下接种于BALB/c裸鼠，并连续进行移植。 [26072］在裸鼠身上的移植过程中，细胞株始终保持结肠癌的原始组织性状。 [26072］在无胸腺小鼠中传代23代后建立了T84细胞株。 这些细胞单层生长到饱和并在接触细胞间展现出紧密连接和桥粒。 [1155] 有很多关于多肽类激素和神经递质并维持定向电解质传输的受体。 [1155］这株细胞展现了接触细胞中的紧密连接和桥粒。 [1155］角蛋白免疫过氧化物酶染色阳性。 T84细胞系常用于人结直肠癌的研究，特别是在研究肠道屏障功能、电解质运输、以及药物吸收和转运方面，通过该细胞可以更好地理解结肠癌的发病机制和寻找新的治疗策略。 注意事项： （1）T84细胞消化传代24-48小时贴壁，细胞消化后48小时后换液操作。 （2）细胞块状生长形态，建议高密度传代。
种属	人
性别/年龄	男/72岁
组织	结肠；来源于转移部位:肺
疾病	大肠癌
细胞类型	肿瘤细胞
形态学	上皮的
生长方式	贴壁
倍增时间	大约33.9小时
培养基和添加剂	DMEM高糖/F-12（中乔新舟  货号：ZQ-600）+5%胎牛血清（中乔新舟  货号：ZQ500-A）+1%P/S（中乔新舟  货号：CSP006） 生长条件： 这株细胞应维持高密度(至少1/4满)生长。
推荐完全培养基货号	ZM0328
生物安全等级	BSL-1
STR位点信息	Amelogenin: X CSF1PO: 10 D13S317: 9 D16S539: 10,11 D5S818: 12 D7S820: 8,10 TH01: 6,9 TPOX: 8,11 vWA: 17,18
培养条件	95%空气，5%二氧化碳；37℃
抗原表达/受体表达	***
基因表达	***
保藏机构	ATCC; CCL-248 ECACC; 88021101
供应限制	仅供科研使用

货号	ZQ0328
发货规格	活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一
发货形式	活细胞：常温运输；冻存管：干冰运输
储存温度	活细胞：培养箱；冻存管：液氮罐
产地	中国
供应限制	仅供科研使用

论文标题： Let-7b ameliorates Crohn’s disease-associated adherent-invasive E coli induced intestinal inflammation via modulating Toll-Like Receptor 4 expression in intestinal epithelial cells
DOI： 10.1016/j.bcp.2018.08.029
发表时间： 2018-08-22
期刊： BIOCHEMICAL PHARMACOLOGY
影响因子： 4.235
货号： ZQ0328
产品名称： T84 cells

原文链接： https://www.sciencedirect.com/science/article/pii/S0006295218303551

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