产品名称	BT-474 [BT474]人乳腺导管癌细胞
货号	ZQ0375
产品介绍	BT-474是一种人类乳腺癌细胞系，来源于一名60岁女性的导管癌。该细胞系雌激素和孕激素受体阳性，使其成为研究激素反应性乳腺癌的有价值的模型。BT-474细胞还以HER2/neu(人表皮生长因子受体2)的过表达为特征，HER2/neu是一种被扩增的蛋白质，在某些侵袭性乳腺癌的发病和进展中起着关键作用。 BT-474细胞系广泛用于肿瘤学研究，研究乳腺癌增殖的分子机制，测试靶向激素受体和HER2通路的治疗策略。这些细胞对于检查her2靶向治疗(如曲妥珠单抗(赫赛汀))的疗效以及探索对这些治疗的耐药机制特别有用。该细胞系还促进了对激素调控如何影响癌细胞生长和存活的理解，为激素依赖性肿瘤的潜在治疗方法提供了见解。 1、该细胞细胞主要是成片生长容易聚团，建议使用多聚-L-赖氨酸溶液(货号：CSP073)包被培养瓶或培养皿。 2、汇合至80%即可传代，细胞不会长太满，5-6天传代一次。
种属	人
性别/年龄	女/60岁
组织	乳腺；乳房/导管
疾病	导管癌
细胞类型	肿瘤细胞
形态学	纺锤形
生长方式	贴壁
倍增时间	大约45.8~100小时
培养基和添加剂	RPMI-1640（中乔新舟 货号：ZQ-200）+10%胎牛血清（中乔新舟  货号：ZQ500-A）+1%P/S（中乔新舟  货号：CSP006）
推荐完全培养基货号	ZM0375
生物安全等级	BSL-1
STR位点信息	Amelogenin: X CSF1PO: 10,11 D13S317: 11 D16S539: 9,11 D5S818: 11,13 D7S820: 9,12 TH01: 7 TPOX: 8 vWA: 15,16
培养条件	95%空气，5%二氧化碳；37℃
抗原表达/受体表达	***
基因表达	***
保藏机构	ATCC; CRL-7913 ATCC; HTB-20 BCRC; 60359 BCRJ; 0353 DSMZ; ACC-64
供应限制	仅供科研使用

货号	ZQ0375
发货规格	活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一
发货形式	活细胞：常温运输；冻存管：干冰运输
储存温度	活细胞：培养箱；冻存管：液氮罐
产地	中国
供应限制	仅供科研使用

论文标题： Prognostic value of gamma-interferon-inducible lysosomal thiol reductase expression in female patients diagnosed with breast cancer
DOI： 10.1002/ijc.33843
发表时间： 2021-10-25
期刊： INTERNATIONAL JOURNAL OF CANCER
影响因子： 7.396
货号： ZQ0375
产品名称： BT-474 cells

原文链接： https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.33843

论文标题： The long noncoding RNA TINCR promotes breast cancer cell proliferation and migration by regulating OAS1
DOI： 10.1038/s41420-021-00419-x
发表时间： 2021-03-01
期刊： Cell Death Discovery
影响因子： 5.241
货号： ZQ0375
产品名称： BT474 cell
原文链接： https://www.nature.com/articles/s41420-021-00419-x

论文标题： Verteporfin inhibits cell proliferation and induces apoptosis in different subtypes of breast cancer cell lines without light activation
DOI： 10.1186/s12885-020-07555-0
发表时间： 2020-10-29
期刊： BMC CANCER
影响因子： 3.15
货号： ZQ0375
产品名称： BT-474 cells
原文链接： https://bmccancer.biomedcentral.com/articles/10.1186/s12885-020-07555-0

论文标题： Determination of the migration effect and molecular docking of verteporfin in different subtypes of breast cancer cells
DOI： 10.3892/mmr.2020.11482
发表时间： 2020-09-02
期刊： Molecular Medicine Reports
影响因子： 2.1
货号： ZQ0375
产品名称： BT-474 cells

原文链接： https://www.spandidos-publications.com/10.3892/mmr.2020.11482

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