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MNNG/HOS Cl #5 [R-1059-D]人骨肉瘤细胞

英文名:MNNG/HOS Cl #5 [R-1059-D]
货号:ZQ0405
价格:¥1600.00
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MNNG/HOS Cl #5 [R-1059-D]人骨肉瘤细胞

¥1600.00
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MNNG/HOS Cl #5 [R-1059-D]人骨肉瘤细胞专用培养基

¥350.00 ¥480.00

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¥1950 ¥2080.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

MNNG/HOS Cl #5 [R-1059-D]人骨肉瘤细胞

货号

ZQ0405

产品介绍

MNNG/HOS Cl #5[R-1059-D]细胞株来源于人骨肉瘤细胞株HOS,经浓度为0.01 mcg/ml的n -甲基-n′-硝基-n -亚硝基胍(MNNG)体外转化。这种化合物是一种强致癌物,这种转化导致了显著的致瘤性,当皮下接种107个细胞时,在21天内以100%的频率在裸鼠中形成肿瘤。这些肿瘤被观察为低分化肉瘤或骨肉瘤。该细胞系最初是从一名13岁的白人女性骨肉瘤患者身上建立的,并表现出粘附生长特性。
在功能上,MNNG/HOS Cl #5细胞在软琼脂中表现出高饱和密度和高镀效率,反映了它们增强的不依赖锚定生长,这是恶性转化的标志。此外,这些细胞表现出显著的纤溶活性,这与增加的致瘤潜力有关。与未经处理的HOS细胞相比,mnng处理的细胞表现出更强大的细胞聚集特性,并且在软琼脂中形成集落的倾向更高,这与它们的肿瘤形成能力相关。在实验中,mnng转化的细胞在裸鼠和仓鼠中均产生肿瘤,其细胞与亲本HOS系相似,而未经处理的细胞在类似条件下无致瘤性。
该细胞系在研究癌症进展和肿瘤生物学方面也很有用,特别是骨肉瘤,因为它提供了化学诱导转化的模型。这些细胞在免疫功能低下的环境(例如,裸鼠)中生长的能力使它们成为临床前癌症研究的宝贵工具,允许研究致瘤机制和潜在的治疗干预测试。

种属

性别/年龄

女/13岁

组织

疾病

骨肉瘤

细胞类型

肿瘤细胞 

形态学

上皮细胞

生长方式

贴壁

倍增时间

大约24小时 

培养基和添加剂

MEM(含NEAA)(品牌:中乔新舟  货号:ZQ-300+10%FBS(品牌:中乔新舟  货号:ZQ500-A+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0405

生物安全等级

BSL-1

STR位点信息

Amelogenin: X

CSF1PO: 12

D13S317: 12

D16S539: 10,13

D5S818: 13

D7S820: 11,12

THO1: 6

TPOX: 8,11

vWA: 18

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ATCC; CRL-1547 ECACC; 87070201

供应限制

仅供科研使用

货号

ZQ0405

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


Amino-Functionalized Zirconium-Based Metal–Organic Frameworks as Bifunctional Nanomaterials to Treat Bone Tumors and Promote Osteogenesis.(2023-11-09)

作者:Jongpeng Yuan,Xujie Liu

期刊:ACS Applied Materials & Interfaces

DOI: 10.1021/acsami.3c11787 

影响因子 9.5

引用产品:MC3T3-E1, MNNG/HOS and MDA-MB-231 cell

https://pubs.acs.org/doi/full/10.1021/acsami.3c11787


论文标题: Ultrafast Low-Temperature Photothermal Therapy Activates Autophagy and Recovers Immunity for Efficient Antitumor Treatment
DOI: 10.1021/acsami.9b19148
发表时间: 2020-01-06
期刊: ACS Applied Materials & Interfaces
影响因子: 8.758
货号: ZQ0405
产品名称: human osteosarcoma cells
原文链接: https://pubs.acs.org/doi/abs/10.1021/acsami.9b19148


论文标题: Circular RNA circVRK1 suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating zinc finger protein ZNF652 expression via microRNA miR-337-3p
DOI: 10.1080/21655979.2021.1965695
发表时间: 2021-08-23
期刊: Bioengineered
影响因子: 3.269
货号: ZQ0405
产品名称: MNNG/HOS cells
原文链接: https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.1965695


论文标题: MicroRNA-506-3p initiates mesenchymal-to-epithelial transition and suppresses autophagy in osteosarcoma cells by directly targeting SPHK1
DOI: 10.1080/09168451.2019.1569496
发表时间: 2019-01-23
期刊: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
影响因子: 1.297
货号: ZQ0405
产品名称: MNNG/HOS cells

原文链接: https://www.tandfonline.com/doi/abs/10.1080/09168451.2019.1569496



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