产品名称	MOLT-4人急性淋巴母细胞白血病细胞
货号	ZQ0429
产品介绍	MOLT-4是一种T淋巴母细胞系，来源于1971年复发的19岁急性淋巴细胞白血病(ALL)男性患者的外周血。它是MOLT-3的姐妹细胞系，而MOLT-4表现出不寻常的t细胞抗原受体γ链基因(t - γ)重排。MOLT-4细胞在未经处理的裸鼠、抗淋巴细胞血清处理的小鼠和x射线照射的小鼠中具有致瘤性，在悬浮中生长。 MOLT-4细胞具有超四倍体染色体数目，模态染色体数目为95，在24%的细胞中发生，但表现出稳定和反复的染色体结构异常和较长的端粒长度。MOLT-4表达多种T细胞标志物，包括CD1、CD2、CD3A、CD3B、CD3C、CD4、CD5、CD6和CD7。它们也表达高水平的末端脱氧核苷酸转移酶(TdT)。MOLT-4细胞系不产生免疫球蛋白或eb病毒。获得细胞的患者先前接受过多药化疗。p53基因密码子248处G -> a突变，p53不表达。该菌株最初被支原体污染，但后来用抗生素去除。 注意：该细胞在1640(含1.5g/LNaHCO3)培养基中生长良好，大部分品牌的1640含有较高浓度的NaHCO3（3.7g/L），若使用1640（3.7g/L NaHCO3）培养基培养细胞时需要提高CO2浓度（7%-10%）。
种属	人
性别/年龄	男/19岁
组织	外周血
疾病	急性淋巴细胞白血病
细胞类型	肿瘤细胞
形态学	成淋巴细胞
生长方式	悬浮
倍增时间	大约24~48小时
培养基和添加剂	RPMI-1640（ATCC改良款）（品牌：中乔新舟 货号：ZQ-200F）+10%FBS（品牌：中乔新舟 货号:ZQ500-A）+1%P/S（中乔新舟  货号：CSP006）
推荐完全培养基货号	ZM0429
生物安全等级	BSL-1
STR位点信息	Amelogenin: XY CSF1PO: 11, 12, 13 D13S317: 12, 13 D16S539: 11, 14 D5S818: 12 D7S820: 8, 10, 11 THO1: 6, 8 TPOX: 8 vWA: 17, 18
培养条件	95%空气，5%二氧化碳；37℃
抗原表达/受体表达	***
基因表达	***
保藏机构	ATCC; CRL-1582 DSMZ; ACC-362 ECACC; 85011413 ECACC; 94022544
供应限制	仅供科研使用

货号	ZQ0429
发货规格	活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一
发货形式	活细胞：常温运输；冻存管：干冰运输
储存温度	活细胞：培养箱；冻存管：液氮罐
产地	中国
供应限制	仅供科研使用

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