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产品名称 |
NK-92人恶性非霍奇金淋巴瘤患者的自然杀伤细胞 |
货号 |
ZQ0434 |
产品介绍 |
NK-92是从一位患有急进性非霍奇金淋巴瘤的50岁白人男性外周血单核细胞衍生来的一株白细胞介素-2依赖型NK细胞株。 这株细胞对很多恶性细胞有细胞毒性;铬释放试验显示它能杀死K562和Daudi细胞。 NK-92细胞(经过照射以防止增殖)可以有效地用于血液中白血病的体外免疫清扫而不危及血细胞的功能。 NK-92细胞有以下特征: CD2, CD7, CD11a, CD28, CD45, CD54表面标记阳性,CD56亮;CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34和HLA-DR表面标记阴性。
注意事项: |
种属 |
人 |
性别/年龄 |
男/50岁 |
组织 |
外周血 |
疾病 |
恶性非霍奇金淋巴瘤 |
细胞类型 |
自然杀伤细胞;nk细胞 |
形态学 |
成淋巴细胞 |
生长方式 |
悬浮;多细胞聚集体 |
倍增时间 |
大约29.5~50小时 |
培养基和添加剂 |
MEMα(中乔新舟 货号:ZQ-800)+12.5%热灭活马血清(中乔新舟 货号:ZQH500)+12.5%进口胎牛(中乔新舟 货号:ZQ0500)+1%双抗(中乔新舟 货号:CSP006)+0.2mM肌醇+0.02mM叶酸+0.1mM β-Mer(中乔新舟 货号:CSP005)+200u/ml重组 IL-2 |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9,12 D16S539: 11,12 D5S818: 12,13 D7S820: 10,11 THO1: 6,9.3 TPOX: 8 vWA: 18 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-2407 DSMZ; ACC-488 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0434 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=8152260
Gong J.-H., Maki G., Klingemann H.-G.
Characterization of a human cell line (NK-92) with phenotypical and functional characteristics of activated natural killer cells.
Leukemia 8:652-658(1994)
PubMed=9118301
Klingemann H.-G., Wong E., Maki G.
A cytotoxic NK-cell line (NK-92) for ex vivo purging of leukemia from blood.
Biol. Blood Marrow Transplant. 2:68-75(1996)
PubMed=9573023; DOI=10.1182/blood.V91.10.3850
Nagashima S., Mailliard R., Kashii Y., Reichert T.E., Herberman R.B., Robbins P., Whiteside T.L.
Stable transduction of the interleukin-2 gene into human natural killer cell lines and their phenotypic and functional characterization in vitro and in vivo.
Blood 91:3850-3861(1998)
PubMed=10365666; DOI=10.1089/10430349950018030
Tam Y.K., Maki G., Miyagawa B., Hennemann B., Tonn T., Klingemann H.-G.
Characterization of genetically altered, interleukin 2-independent natural killer cell lines suitable for adoptive cellular immunotherapy.
Hum. Gene Ther. 10:1359-1373(1999)
PubMed=10803505; DOI=10.1038/sj.leu.2401778
Drexler H.G., Matsuo Y.
Malignant hematopoietic cell lines: in vitro models for the study of natural killer cell leukemia-lymphoma.
Leukemia 14:777-782(2000)
DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001)
PubMed=11454312; DOI=10.1089/152581601750288975
Maki G., Klingemann H.-G., Martinson J.A., Tam Y.K.
Factors regulating the cytotoxic activity of the human natural killer cell line, NK-92.
J. Hematother. Stem Cell Res. 10:369-383(2001)
PubMed=12850795; DOI=10.1080/14653240310001523
Tam Y.K., Martinson J.A., Doligosa K., Klingemann H.-G.
Ex vivo expansion of the highly cytotoxic human natural killer-92 cell-line under current good manufacturing practice conditions for clinical adoptive cellular immunotherapy.
Cytotherapy 5:259-272(2003)
PubMed=16827800; DOI=10.1111/j.1349-7006.2006.00226.x
Liu A., Takakuwa T., Luo W.-J., Fujita S., Aozasa K.
Alterations in ATR in nasal NK/T-cell lymphoma and chronic active Epstein-Barr virus infection.
Cancer Sci. 97:605-610(2006)
PubMed=18424763; DOI=10.4049/jimmunol.180.9.6392
Binyamin L., Alpaugh R.K., Hughes T.L., Lutz C.T., Campbell K.S., Weiner L.M.
Blocking NK cell inhibitory self-recognition promotes antibody-dependent cellular cytotoxicity in a model of anti-lymphoma therapy.
J. Immunol. 180:6392-6401(2008)
PubMed=19194464; DOI=10.1038/leu.2009.3
Iqbal J., Kucuk C., Deleeuw R.J., Srivastava G., Tam W., Geng H., Klinkebiel D.L., Christman J.K., Patel K., Cao K., Shen L., Dybkaer K., Tsui I.F.L., Ali H.H., Shimizu N., Au W.Y., Lam W.L., Chan W.C.
Genomic analyses reveal global functional alterations that promote tumor growth and novel tumor suppressor genes in natural killer-cell malignancies.
Leukemia 23:1139-1151(2009)
PubMed=20454443; DOI=10.1155/2010/904767
Uphoff C.C., Denkmann S.A., Steube K.G., Drexler H.G.
Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in human and other primate cell lines.
J. Biomed. Biotechnol. 2010:904767.1-904767.23(2010)
PubMed=21052088; DOI=10.1038/leu.2010.255
Iqbal J., Weisenburger D.D., Chowdhury A., Tsai M.Y., Srivastava G., Greiner T.C., Kucuk C., Deffenbacher K., Vose J.M., Smith L., Au W.Y., Nakamura S., Seto M., Delabie J., Berger F., Loong F., Ko Y.-H., Sng I., Liu X., Loughran T.P. Jr., Armitage J.O., Chan W.C.
Natural killer cell lymphoma shares strikingly similar molecular features with a group of non-hepatosplenic gammadelta T-cell lymphoma and is highly sensitive to a novel aurora kinase A inhibitor in vitro.
Leukemia 25:348-358(2011)
PubMed=21570725; DOI=10.1016/j.molimm.2011.04.010
Schnueriger A., Grau R., Sondermann P., Schreitmueller T., Marti S., Zocher M.
Development of a quantitative, cell-line based assay to measure ADCC activity mediated by therapeutic antibodies.
Mol. Immunol. 48:1512-1517(2011)
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