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人乙型肝炎定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Hepatitis B qPCR Array Kit (GQH-HPB) is designed to facilitate gene expression profiling of 88 key genes involved in hepatitis B pathogenesis. Hepatitis B is a liver infectious disease caused by the Hepatitis B virus (HBV). The HBV infection of hepatocytes triggers immune response cascades to regulate HBV replication and hepatocyte survival. Brief examples of how included genes may be grouped are shown below:
HBV binding factor: HSPG2, CPD
Signaling pathways
MAPK: MAPK1, MAP3K1, MAPK8, MAPK10, MAP2K1, RAF1, ELK1, YWHAB, YWHAQ, FOS
PI3K/AKT: AKT1, PIK3CA, PIK3CG, PIK3R1, PIK3R3, PIK3R5
Toll-like receptor: TLR2, TLR3, TLR4, IFNAs, IFNAR1, IFNB1
JAK/STAT: STAT1, STAT3, JAK1, MMP9, NFKB1, RELA, SRC
Genes implicated in HBV infection: LAMTOR5, RSF1, APOBEC3G, GPT, TNF, AIP, AFP, MBL2, F2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Hepatitis B qPCR Array Plate Layout*(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AFP |
ATP6AP1 |
DDX58 |
HSPG2 |
IFNA2 |
IFNB1 |
MAP2K4 |
MRC1 |
PTMA |
SRC |
TIRAP |
β-actin |
|
B |
AIP |
CPD |
EGR3 |
IFIH1 |
IFNA21 |
IFNG |
MAP3K1 |
NFKB1 |
RAF1 |
STAT1 |
TLR2 |
GAPDH |
|
C |
AKT1 |
CREB3 |
ELK1 |
IFNA1 |
IFNA4 |
IKBKE |
MAPK1 |
PIK3CA |
RARB |
STAT3 |
TLR3 |
LDHA |
|
D |
ALB |
CREB3L1 |
F2 |
IFNA10 |
IFNA5 |
IRF3 |
MAPK10 |
PIK3CG |
RELA |
STAT6 |
TLR4 |
NONO |
|
E |
APOBEC3G |
CREB3L2 |
FGL2 |
IFNA13 |
IFNA6 |
JAK1 |
MAPK8 |
PIK3R1 |
RFX1 |
TGFB1 |
TNF |
PPIH |
|
F |
ASGR1 |
CTLA4 |
FOS |
IFNA14 |
IFNA7 |
LAMTOR5 |
MAVS |
PIK3R3 |
RSF1 |
TGFBR1 |
VDAC3 |
GDC |
|
G |
ATF4 |
DDB2 |
GGT1 |
IFNA16 |
IFNA8 |
MAP2K1 |
MBL2 |
PIK3R5 |
SLC17A5 |
TICAM1 |
YWHAB |
PPC |
|
H |
ATF6B |
DDX3X |
GPT |
IFNA17 |
IFNAR1 |
MAP2K2 |
MMP9 |
PSMA7 |
SON |
TICAM2 |
YWHAQ |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK043 |
|
产地 |
美国 |
|
缩写 |
GQH-HPB |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
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