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人先天性免疫应答定量PCR芯片试剂盒
英文名:GeneQuery™ Human Innate Immune Response qPCR Array Kit
货号:GK039
价格:¥4320.00
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  • 产品规格
  • 参考文献
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人先天性免疫应答定量PCR芯片试剂盒描述:



       ScienCell's GeneQuery™ Human Innate Immune Response qPCR Array Kit (GQH-IIR) facilitates gene expression profiling of key genes in the human innate immune system. The innate immune response is activated upon microbial infection or foreign substance presentation and includes activation of the complement system and adaptive immune systems. A separate array is available for analyzing the adaptive immune response. Brief examples of how included genes may be grouped according to function are shown below:


       Bacteria response: TLR1, TLR5, NOD1, LY96, TRAM1

       Virus response: TLR3, DDX58, IFIH1, MYD88, IRAK4

       Self recognition: TLR2, TLR4, TLR7, TLR8, CLEC4E

       Inflammasome component: CASP1, CASP5, PYCARD, NLRP1

       Complement system component: C3, MBL2, MASP1, CFB, CD46


       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.


       GeneQuery™ Human Innate Immune Response qPCR Array Plate Layout* (8 controls in Bold and Italic)



 

1

2

3

4

5

6

7

8

9

10

11

12

A

ATF3

CASP5

CLEC6A

IFIH1

IRAK1

LYZ

NAP1L1

OTUD5

TICAM1

TLR5

TRAF6

β-actin

B

C3

CD46

CLEC7A

IFNA1

IRAK2

MAP3K7

NFAM1

PYCARD

TICAM2

TLR6

TRAM1

GAPDH

C

C5

CFB

DDX58

IFNB1

IRAK3

MAPK8

NFATC1

RIPK2

TIRAP

TLR7

TRAM2

LDHA

D

C6

CFD

DHX58

IFNG

IRAK4

MASP1

NFKB1

RNF125

TLR1

TLR8

TRIM25

NONO

E

C7

CFH

EYA4

IL18

IRF3

MASP2

NFKBIE

SYK

TLR10

TLR9

TYROBP

PPIH

F

C8A

CFP

FADD

IL1A

IRF7

MAVS

NLRP1

TAB2

TLR2

TNF

UBE2N

GDC

G

CARD9

CHUK

HCST

IL1B

JUN

MBL2

NOD1

TAB3

TLR3

TRADD

UBE2V1

PPC

H

CASP1

CLEC4E

HMGB1

IL6

LY96

MYD88

NOD2

TBK1

TLR4

TRAF3

UNC93B1

NTC


 *gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用

货号

GK039

产地

美国

缩写

GQH-IIR

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

       [1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12.
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