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人细胞周期定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ human cell cycle qPCR array kit (GQH-CCY) is designed to facilitate gene expression profiling of key genes involved in human cell cycle progression. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Cell Cycle qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ANAPC2 |
BUB3 |
CCND2 |
CDC20 |
CDK6 |
CHEK1 |
E2F1 |
HDAC1 |
MCM3 |
RAD1 |
SMAD2 |
β-actin |
|
B |
ATM |
CASP3 |
CCND3 |
CDC25A |
CDK7 |
CHEK2 |
E2F2 |
HDAC2 |
MCM4 |
RAD17 |
SMC3 |
GAPDH |
|
C |
ATR |
CCNA1 |
CCNE2 |
CDC25C |
CDK8 |
CKS1B |
E2F4 |
HUS1 |
MCM5 |
RAD51 |
STAG1 |
LDHA |
|
D |
AURKB |
CCNA2 |
CCNF |
CDC34 |
CDKN1A |
CKS2 |
E2F5 |
KNTC1 |
MDM2 |
RAD9A |
TFDP1 |
NONO |
|
E |
BCCIP |
CCNB1 |
CCNG1 |
CDC6 |
CDKN1B |
CUL1 |
EP300 |
KPNA2 |
MYC |
RB1 |
TFDP2 |
PPIH |
|
F |
BIRC5 |
CCNB2 |
CCNH |
CDK1 |
CDKN2A |
CUL2 |
GADD45A |
MAD2L1 |
NBN |
RBL1 |
TGFB1 |
GDC |
|
G |
BRCA1 |
CCNC |
CCNT1 |
CDK2 |
CDKN2B |
CUL3 |
GSK3B |
MAD2L2 |
ORC1 |
RBL2 |
TP53 |
PPC |
|
H |
BRCA2 |
CCND1 |
CDC16 |
CDK4 |
CDKN3 |
DBF4 |
GTSE1 |
MCM2 |
PCNA |
SKP2 |
WEE1 |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK003 |
|
产地 |
美国 |
|
缩写 |
GQH-CCY |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
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