小鼠IL-3ELISA试剂盒描述:
The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant mouse IL-3, consisting of 135 amino acids with the molecular mass of 15 KDa. ScienCell's mouse IL-3 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse IL-3-specific polyclonal antibodies are precoated onto 8 x 12 strips. The mouse specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse IL-3 in the sample that is captured in the strips.
产品使用说明:仅供科研研究使用
货号 |
EK0403 |
产地 |
美国 |
缩写 |
m IL-3 -ELISA |
规格 |
96 tests |
用途 |
科研 |
保存 |
频繁使用存储在4°C,很少使用存储在-20°C |
运输 |
胶冰 |
参考文献 |
1. Le Beau, M. M.; Epstein, N. D.; O'Brien,
S. J.; Nienhuis, A. W.; Yang, Y.-C.; Clark, S. C.; Rowley, J. D. The
interleukin 3 gene is located on human chromosome 5 and is deleted in myeloid
leukemias with a deletion of 5q. Proc. Nat. Acad. Sci. 84: 5913-5917, 1987.
2. Clark-Lewis, I.; Aebersold, R.; Ziltener,
H.; Schrader, J. W.; Hood, L. E.; Kent, S. B. H. Automated chemical synthesis
of a protein growth factor for hemopoietic cells, interleukin-3. Science 231:
134-139, 1986.
3. Fung, M. C.; Hapel, A. J.; Ymer, S.;
Cohen, D. R.; Johnson, R. M.; Campbell, H. D.; Young, I. G. Molecular cloning
of cDNA for murine interleukin-
3. Nature 307: 233-237, 1984.
4. Ihle, J. N.; Silver, J.; Kozak, C. A.
Genetic mapping of the mouse interleukin 3 gene to chromosome 11. J. Immun.
138: 3051-3054, 1987.
5. Yang, Y.-C.; Kovacic, S.; Kriz, R.; Wolf,
S.; Clark, S. C.; Wellems, T. E.; Nienhuis, A.; Epstein, N. The human genes
for GM-CSF and IL3 are closely linked in tandem on chromosome 5. Blood 71:
958-961, 1988.
|
1. Le Beau, M. M.; Epstein, N. D.; O'Brien, S. J.; Nienhuis, A. W.; Yang, Y.-C.; Clark, S. C.; Rowley, J. D. The interleukin 3 gene is located on human chromosome 5 and is deleted in myeloid leukemias with a deletion of 5q. Proc. Nat. Acad. Sci. 84: 5913-5917, 1987.
2. Clark-Lewis, I.; Aebersold, R.; Ziltener, H.; Schrader, J. W.; Hood, L. E.; Kent, S. B. H. Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3. Science 231: 134-139, 1986.
3. Fung, M. C.; Hapel, A. J.; Ymer, S.; Cohen, D. R.; Johnson, R. M.; Campbell, H. D.; Young, I. G. Molecular cloning of cDNA for murine interleukin-
3. Nature 307: 233-237, 1984.
4. Ihle, J. N.; Silver, J.; Kozak, C. A. Genetic mapping of the mouse interleukin 3 gene to chromosome 11. J. Immun. 138: 3051-3054, 1987.
5. Yang, Y.-C.; Kovacic, S.; Kriz, R.; Wolf, S.; Clark, S. C.; Wellems, T. E.; Nienhuis, A.; Epstein, N. The human genes for GM-CSF and IL3 are closely linked in tandem on chromosome 5. Blood 71: 958-961, 1988.
产品名称 | 价格 | 指令 |
小鼠促红细胞生成素ELISA试剂盒 | ¥7560.00 | 购物车 》 |
小鼠IGFBP-3ELISA试剂盒 | ¥6660.00 | 购物车 》 |
小鼠MMP-3ELISA试剂盒 | ¥6660.00 | 购物车 》 |
小鼠血管细胞粘附分子-1ELISA试剂盒 | ¥5940.00 | 购物车 》 |
小鼠IL-23ELISA试剂盒 | ¥7020.00 | 购物车 》 |
大鼠Neurotrophin-3ELISA试剂盒 | ¥5265.00 | 购物车 》 |